In Situ Hybridisation: Application to Developmental Biology and Medicine

doi:10.1017/CBO9780511629051

Advances in our understanding of biological mechanisms have frequently been associated with the development of techniques. In situ hybridisation is a classic case of just such an advance. The technique effectively combines histochemistry with molecular biology and enables the rapid analysis of the distribution of RNA, or DNA, in the tissues. The information gained from this has caused something of a revolution in our understanding of developmental biology, since a fundamental aspect of development is the spatial and temporal expression of genes. In addition the technique has found application in the field of medicine, providing insights into the functioning of healthy tissues and the diagnosis and study of diseases. This book brings together contributions from leaders in the application of in situ hybridisation and guides the would-be exponent through the various options and variations of the technique.

"...very useful for biologists, molecular biologists, biophysicists, and advanced students." Journal of Steroid Biochemistry and Molecular Biology

- Aa Reduce text
- Aa Enlarge text

View selected items
Save to my bookmarks
Export citations
Download PDF (zip)
Save to Kindle
Save to Dropbox
Save to Google Drive
Save content to
To save content items to your account, please confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your account. Find out more about saving content to .

To save content items to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about saving to your Kindle.

Note you can select to save to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be saved to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

Find out more about the Kindle Personal Document Service.

Please be advised that item(s) you selected are not available.
You are about to save
Your Kindle email address

Please provide your Kindle email.

@free.kindle.com @kindle.com (service fees apply)

By using this service, you agree that you will only keep content for personal use, and will not openly distribute them via Dropbox, Google Drive or other file sharing services

Contents

Frontmatter
pp i-iv
- - Get access
    
    Check if you have access via personal or institutional login
    
    Log in Register
- Export citation

Contents
pp v-vi
- - Get access
    
    Check if you have access via personal or institutional login
    
    Log in Register
- Export citation

List of contributors
pp vii-x
- - Get access
    
    Check if you have access via personal or institutional login
    
    Log in Register
- Export citation

Preface
pp xi-xii
- - Get access
    
    Check if you have access via personal or institutional login
    
    Log in Register
- Export citation

Non-radioisotopic labels for in situ hybridisation histochemistry: a histochemist's view.
pp 1-32
- By Gary Coulton
- - Get access
    
    Check if you have access via personal or institutional login
    
    Log in Register
- Export citation

Use of haptenised nucleic acid probes in fluorescent in situ hybridisation
pp 33-42
- By A. K. Raap, P. M. Nederlof, R. W. Dirks, J. C. A. G. Weigant, M. van der Ploeg
- - Get access
    
    Check if you have access via personal or institutional login
    
    Log in Register
- Export citation

The use of complementary RNA probes for the identification and localisation of peptide messenger RNA in the diffuse neuroendocrine system
pp 43-68
- By A. Giaid, S. J. Gibson, J. H. Steel, P. Facer, J. M. Polak
- - Get access
    
    Check if you have access via personal or institutional login
    
    Log in Register
- Export citation

Contributions of the spatial analysis of gene expression to the study of sea urchin development
pp 69-96
- By Robert C. Angerer, Susan D. Reynolds, Julia Grimwade, David L. Hurley, Qing Yang, Paul D. Kingsley, Michael L. Gagnon, James Palis, Lynne M. Angerer
- - Get access
    
    Check if you have access via personal or institutional login
    
    Log in Register
- Export citation

Advantages and limitations of in situ hybridisation as exemplified by the molecular genetic analysis of Drosophila development
pp 97-114
- By P. W. Ingham, A. Hidalgo, A. M. Taylor
- - Get access
    
    Check if you have access via personal or institutional login
    
    Log in Register
- Export citation

The use of in situ hybridisation to study the localisation of maternal mRNAs during Xenopus oogenesis
pp 115-130
- By H. Perry-O'Keefe, C. R. Kintner, J. Yisraeli, D. A. Melton
- - Get access
    
    Check if you have access via personal or institutional login
    
    Log in Register
- Export citation

In situ hybridisation in the analysis of genes with potential roles in mouse embryogenesis
pp 131-142
- By David G. Wilkinson
- - Get access
    
    Check if you have access via personal or institutional login
    
    Log in Register
- Export citation

Evolution of algal plastids from eukaryotic endosymbionts
pp 143-156
- By Geoffrey Ian McFadden
- - Get access
    
    Check if you have access via personal or institutional login
    
    Log in Register
- Export citation

Localisation of expression of male flower-specific genes from maize by in situ hybridisation
pp 157-174
- By Susan Y. Wright, Andrew J. Greenland
- - Get access
    
    Check if you have access via personal or institutional login
    
    Log in Register
- Export citation

Tissue preparation techniques for in situ hybridisation studies of storage-protein gene expression during pea seed development
pp 175-188
- By N. Harris, J. Mujcrone, H. Grindley
- - Get access
    
    Check if you have access via personal or institutional login
    
    Log in Register
- Export citation

Investigation of gene expression during plant gametogenesis by in situ hybridisation
pp 189-204
- By K. G. Jones, S. J. Crossley, H. G. Dickinson
- - Get access
    
    Check if you have access via personal or institutional login
    
    Log in Register
- Export citation

Sexing the human conceptus by in situ hybridisation
pp 205-240
- By John D. West
- - Get access
    
    Check if you have access via personal or institutional login
    
    Log in Register
- Export citation

Non-isotopic in situ hybridisation in human pathology
pp 241-270
- By C. S. Herrington, J. Burns, J. O'D. McGee
- - Get access
    
    Check if you have access via personal or institutional login
    
    Log in Register
- Export citation

The demonstration of viral DNA in human tissues by in situ DNA hybridisation
pp 271-284
- By M. Wells
- - Get access
    
    Check if you have access via personal or institutional login
    
    Log in Register
- Export citation

Index
pp 285-288
- - Get access
    
    Check if you have access via personal or institutional login
    
    Log in Register
- Export citation

Metrics

Total number of HTML views: 0

Total number of PDF views: 0 *

Loading metrics...

Total views: 0 *

Loading metrics...

* Views captured on Cambridge Core between #date#. This data will be updated every 24 hours.

Usage data cannot currently be displayed.

Why this information is here

This section outlines the accessibility features of this content - including support for screen readers, full keyboard navigation and high-contrast display options. This may not be relevant for you.

Accessibility Information

Accessibility compliance for the PDF of this book is currently unknown and may be updated in the future.

In Situ Hybridisation

Application to Developmental Biology and Medicine

This Book has been cited by the following publications. This list is generated based on data provided by Crossref.

Book description

Reviews

Refine List

Actions for selected content:

Contents

Frontmatter
pp i-iv

Contents
pp v-vi

List of contributors
pp vii-x

Preface
pp xi-xii

Non-radioisotopic labels for in situ hybridisation histochemistry: a histochemist's view.
pp 1-32

Use of haptenised nucleic acid probes in fluorescent in situ hybridisation
pp 33-42

The use of complementary RNA probes for the identification and localisation of peptide messenger RNA in the diffuse neuroendocrine system
pp 43-68

Contributions of the spatial analysis of gene expression to the study of sea urchin development
pp 69-96

Advantages and limitations of in situ hybridisation as exemplified by the molecular genetic analysis of Drosophila development
pp 97-114

The use of in situ hybridisation to study the localisation of maternal mRNAs during Xenopus oogenesis
pp 115-130

In situ hybridisation in the analysis of genes with potential roles in mouse embryogenesis
pp 131-142

Evolution of algal plastids from eukaryotic endosymbionts
pp 143-156

Localisation of expression of male flower-specific genes from maize by in situ hybridisation
pp 157-174

Tissue preparation techniques for in situ hybridisation studies of storage-protein gene expression during pea seed development
pp 175-188

Investigation of gene expression during plant gametogenesis by in situ hybridisation
pp 189-204

Sexing the human conceptus by in situ hybridisation
pp 205-240

Non-isotopic in situ hybridisation in human pathology
pp 241-270

The demonstration of viral DNA in human tissues by in situ DNA hybridisation
pp 271-284

Index
pp 285-288

Metrics

Full text views

Book summary page views

Accessibility standard: Unknown

Why this information is here

Accessibility Information

In Situ Hybridisation

Application to Developmental Biology and Medicine

Book description

Reviews

Refine List

Actions for selected content:

Save Search

Contents

Metrics

Full text views

Book summary page views

Accessibility standard: Unknown

Why this information is here

Accessibility Information