To explore molecular targets for regulating glucose metabolism in carnivorous fish, the glucose tolerance test (GTT) was carried out on the Paralichthys olivaceus. The concentration of glucose and insulin in serum were measured at 0, 1, 3, 5, 7, 9, 12, 24, and 48h after intraperitoneal (IP) injecting. The concentration of insulin was the lowest after 3h of glucose injection, and that of glucose reached the highest after 5h. Therefore, 0h (IP0) was chosen as control group, 3h (IP3) and 5h (IP5) were selected as experimental groups, which the liver samples in three time points were used to high-throughput sequencing. Although, there were no significant KEGG and GO functional enrichment, the differential genes including MAPK binding protein 1 (MAPKBP1), glycosyltransferase (FNG), suppressor of cytokine signaling 3 (SOCS3), CCAAT/enhancer-binding protein alpha (CEBP-α) were closely related to glucose metabolism, among which SOCS3 was worthy of further explore. The full-length cDNA sequence of SOCS3 gene was cloned and the open reading frame (ORF) of SOCS3 encoded 225 amino acids including conserved domains SH2 and SOCS3-box. The results of tissue differential expression showed that SOCS3 was highly expressed in liver and intestine. The SOCS3 was knocked down by specific siRNA in the primary hepatocyte of P. olivaceus. Results showed that the gene expression of insulin receptor substrate 1 (IRS1), protease B1 (AKT1), glucose transporter 2 (GLUT2), pyruvate kinase (PK) and glucokinase (GK) increased significantly after knocking down SOCS3. Meanwhile, the phosphatidylinositol-3-hydroxykinase (PI3K) and glucose-6-phosphatase (G6Pase) decreased significantly. The results of this study indicated that siSOSC3 enhanced the sensitivity of the insulin signaling pathway to promote glucose transport, thereby affecting gluconeogenesis and glycolysis to maintain glucose homeostasis.

The effects arising from interactions between two identical starting jets on their propulsive characteristics have been investigated numerically for different dimensionless distances $S/D$ (the distance between two jet axes normalised by nozzle diameter, from 1.1 to 4) and stroke ratios $L/D$ (the length-to-diameter ratio of jet column, from 2 to 5). The two jets are arranged in parallel and initiated simultaneously with identical conditions. Their leading vortical structures evolve from an axisymmetric to a plane-symmetric configuration, with deceleration in regions where the two jet wakes approach each other. The generation of axial thrust is affected, primarily dominated by variations in the pressure thrust component. This results from the combination of the mutually induced pressure (MIP) and the coupling effects of vortex rings (CEVR for $S/D\gt1.5$ and CEVR-R for $S/D\lt1.5$). The MIP governs the fluctuations introduced into thrust development, while CEVR (CEVR-R) is responsible for the reduction in average thrust. These effects become more pronounced as $S/D$ decreases, but remain almost unaffected by $L/D$. Adjusting the acceleration and deceleration rates of the velocity program shows limited effects on either the thrust fluctuations or the average thrust reduction. Furthermore, the interaction induces two lateral force components with equal magnitude but opposite directions on the outer walls of the two nozzles, with their magnitude exceeding $15\,\%$ of the axial thrust. The introduction of an additional vertical wall within the nozzle exit plane effectively eliminates the lateral force. However, it consequently enhances both the average thrust reduction and the thrust fluctuations induced by the interaction.

Understanding spatial navigation and memory formation is critical to exploring how humans learn and adapt in complex environments. To investigate these processes, we conducted an experiment using the Minecraft Memory and Navigation Task, collecting detailed three-dimensional (3D) path data in a virtual open-world setting. Statistically, we developed a novel methodology to convert complex high-dimensional 3D movement data into functional representations, enabling standardized comparisons and analyses across participants and environments. We applied techniques such as functional clustering and regression to identify navigation patterns and their relationships with cognitive map development and memory retention. Our analysis uncovered two significant insights: first, participants who adopted moderately exploratory behaviors during training demonstrated superior retention of object locations; second, inefficient navigation strategies were strongly linked to poorer spatial memory and navigation performance. These findings highlight the effectiveness of our methodology in advancing the study of navigation behaviors and cognitive processes in dynamic 3D environments.

We present an experimental study of proton acceleration driven by femtosecond multi-PW lasers of three different prepulse parameters with the peak laser intensity of 1.2 × 1021 W/cm2 irradiating micrometre-thick metal foils. For 4-μm-thick copper foils, the highest-energy proton beam of 58.9 MeV is generated with the moderate-contrast laser, while the low-contrast or high-contrast lasers result in the lower proton cutoff energies. The one-dimensional hydrodynamic and two-dimensional particle-in-cell simulations indicate that the front preplasma of foils induced by the laser prepulse can enhance electron acceleration and in turn improve proton acceleration, while the rear preplasma will weaken the sheath field and be unfavourable for accelerating ions. For the case of the moderate contrast, the scale length of the front preplasma is long enough to generate high-temperature electrons compared to the high-contrast case, and the scale length of the rear preplasma is so short that the sheath field still remains strong compared with the low-contrast case, which is advantageous for generating high-energy protons. Meanwhile, a concrete map is theoretically given for accelerating higher-energy protons. This work extends the concept of the prepulse effect on target normal sheath acceleration (TNSA) to a wider range of laser parameters (multi-PW, 1021 W/cm2), representing an important step towards potential applications of TNSA-driven proton sources, especially considering that PW and even 10 PW laser facilities exist all around the world.

The compression waves/boundary layer interaction (CWsBLI) in high-speed inlets poses significant challenges for predicting flow separation, rendering traditional shock wave/boundary layer interaction (SWBLI) scaling laws inadequate due to unaccounted effects of the coverage range of compression waves. This study aims to establish a unified scaling framework for CWsBLIs and SWBLIs by proposing an equivalent interaction intensity. Experiments were conducted in a Mach 2.5 supersonic wind tunnel, employing schlieren imaging and pressure measurements to characterise flows induced by curved surfaces at two deflection angles ($10^{\circ }, 12^{\circ }$) and varying coverage ranges of compression waves ($d$). An equivalent transformation method was developed to convert the CWsBLI into an equivalent incident SWBLI (ISWBLI), with interaction intensity derived from pressure gradients considering the coverage range. Key results reveal a critical threshold based on the interaction length of ISWBLI ($L_{\textit{single}}$): when $d \leq L_{\textit{single}}$, the interaction scale remains comparable to ISWBLI; when $d \gt L_{\textit{single}}$, the weakened adverse pressure gradient leads to a reduction in the length scale. The proposed scaling framework unifies the CWsBLIs and SWBLIs, achieving better data collapse compared to the existing methods. This work advances our understanding of complex waves/boundary layer interactions, and provides a prediction method for the length scales of CWsBLIs.

The dual-tone transition phenomenon and its formation mechanism in the flow around a heptagonal cylinder (side number $N= 7$) are experimentally investigated in depth over a range of free-stream velocities corresponding to Reynolds numbers of the order of $10^4$–$10^5$. Dual tone in this context refers to the emergence of two dominant peaks in the far-field acoustic spectrum when a flow in transition regime passes over a polygonal cylinder in principal orientations. The dual-tone phenomenon is also observed in an $N = 9$ cylinder in the face orientation and an $N = 11$ in the corner orientation, which contrasts with all the other polygonal cylinders of $N\in {\mathbb{Z}}[3,12]$ systematically investigated in the present study, where only a single tonal peak dominates the spectrum, similar to the Aeolian tone observed in the circular cylinder in the subcritical regime. The emergence of the dual tone is found to be responsible for the reduction of far-field noise. Continuous wavelet transform analysis reveals that the occurrence of the two competing tones in the time domain can be empirically modelled by Gaussian distributions. Additional proper orthogonal decomposition based phase averaging using time-resolved planar particle image velocimetry enables coherent vortex structure identification for the two quasi-stable shedding modes, which are responsible for the formation of the two tones. Near-wall flow and pressure fluctuation analysis further confirms that the two tones originate from stochastic shear-layer separation–reattachment switching, thereby generating two patterns of dipole sound sources through distinct vortex formation pathways.

Given its ubiquitous nature, sport events are one of the most popular venues for episodic volunteering around the world. This article explores the rare dataset of volunteering in sport events in seven countries—Finland, Ghana, India, Japan, Switzerland, Tanzania, United States—to overview the differences and similarities identified in demography, volunteers’ reactions to hosts’ management strategies, satisfaction, and intention to volunteer again. Key findings include differences in the level of satisfaction, where responses among American volunteers were the highest. We also find that although well-organized management strategies such as quality training, communication, and proper appreciation led to higher satisfaction, these factors did not contribute to volunteers’ intent to volunteer again. Such management style might be too impersonal with less autonomy on the part of volunteers that they may not feel needed in future events. Somewhat messy management might motivate volunteers to participate again.

In this paper, we numerically investigate the orbit dynamics of three-dimensional symmetric Janus drops in shear flow using an improved ternary-fluids phase field method, focusing on how drop deformation and initial orientation affect the orbit drift of two configurations of Janus drops: dumbbell-shaped and near-spherical. We find that the motion of dumbbell-shaped drops eventually evolves into tumbling, while near-spherical drops attain stable spinning. We attribute this bifurcation in orbit drift to contrasting deformation dynamics and shape-dependent hydrodynamics of the two configurations. Specifically, the drift bifurcation is closely related to the aspect ratio of Janus drops at equilibrium, giving rise to two distinct mechanisms: (1) coupling between outer interface deformation and the surrounding flow field; and (2) interplay between inner interface deformation and vortices enclosed within the drop. In addition, we observe that for the dumbbell-shaped Janus drops with different aspect ratios, their tumbling dynamics resembles ellipsoids in shear flow. Moreover, the trajectories of the dumbbell-shaped Janus drops during orbit drift collapse onto a universal curve, independent of their initial orientations, and significant deformation and inertia accelerate the orbit transition. To quantitatively evaluate the effect of drop deformation on the orbit drift of the dumbbell-shaped Janus drops, we propose an effective aspect ratio model based on the drop shapes at equilibrium and at the maximum elongation. By incorporating the effective aspect ratio into Jeffery’s theory for solid particles, we accurately predict the rotation period and angular velocity of Janus drops in the tumbling regime and during the orbit drift, especially for drops with linear deformation. Moreover, the orbit parameter $C$ is found to vary exponentially with time for drops with linear deformation, while the time variation of $C$ transits from one exponential function to another for drops with nonlinear deformation.

Bemisia tabaci is one of the most important agricultural pests worldwide, and the combined application of multiple natural enemies such as predators and parasitoids can potentially control B. tabaci. The study examined whether the predator Orius similis and the parasitoid Encarsia formosa can synergistically control B. tabaci (crop: kidney bean). The greenhouse cage method was used to release O. similis and E. formosa alone or in combination in different ratios. The combined release of O. similis and E. formosa synergistically decreased the B. tabaci population when compared with O. similis or E. formosa alone. Additionally, O. similis + E. formosa decreased the number of E. formosa black pupae and adults in each crop stage. However, the niche overlap index of E. formosa with B. tabaci nymphs in the O. similis + E. formosa group was higher than in the E. formosa group. Grey correlation analysis revealed that the correlation degree between natural enemies and B. tabaci was the highest when the O. similis and E. formosa release ratio was 1:3. These findings indicate that the combined release of O. similis and E. formosa synergistically controlled B. tabaci with the release ratio 1:3 being optimal for field application.

This paper aims to theorise how virtual reality (VR) can contribute to the development of contextual architecture. We start by considering how an architectural context may translate into a virtual domain, introducing preliminary definitions of what a virtual design context (VDC) could entail. We then discuss a proposed taxonomy that guides the creation of such a VDC, anchored in principles drawn from virtual realism in art philosophy and contextualism within architecture. This taxonomy is envisioned as a preliminary framework for developing VR-driven design environments with a focus on context. Next, we conducted expert user-testing with 24 architects using two VDCs developed according to the taxonomy. The goal of this step was to gain insights regarding the cognitive load of designers and their user experience while engaged in different types of VDCs. Results suggest that designing in these virtual environments enhanced contextual learning, supported conceptual and creative insight and helped maintain manageable cognitive load. The paper concludes by underscoring the real-world applicability of this taxonomy, highlighting how VR can breathe new life into contextual design, not by reducing context into a digital replica, but by opening new dimensions through which its richness can be explored, interpreted and reimagined.

This study uses a coupled lattice Boltzmann and discrete element method to perform interface-resolved simulations of turbulent channel flow laden with finite-size cylindrical particles. The aim is to investigate interactions between wall-bounded turbulence and non-spherical particles with sharp edges. The particle-to-fluid density ratio is unity and gravity is neglected. Comparative analyses are conducted among long (length-to-diameter aspect ratio 2), unit (1) and short ($ 1/2 $) cylinders, along with spheres and literature data for spheroids. Results reveal both shared and distinct dynamic behaviours of cylinders and their effects on turbulence modulation. Notably, disk-like short cylinders can remain trapped near the wall due to their flat faces aligning closely with it – a behaviour unique to particles with sharp edges. Long and unit cylinders, as well as spheres, preferentially accumulate in high-speed streaks, while short cylinders cluster in low-speed streaks, demonstrating a strong aspect-ratio effect. Near the wall, long cylinders align their axis with the streamwise direction, while short cylinders orient perpendicular to the wall. Rotationally, long cylinders primarily spin, whereas short ones predominantly tumble. These trends arise from orientation preferences and differences in axial and spanwise moments of inertia. Cylindrical particles increase wall drag compared with the single-phase case, with short cylinders causing the greatest enhancement due to strong near-wall accumulation. Overall, the influence of aspect ratio on particle dynamics and turbulence modulation is more pronounced for cylindrical particles than for spheroidal ones.