Probiotics represent a promising alternative to antibiotics in livestock production. This study investigated the effects of compound probiotic fermentation (FAM, comprising Lactobacillus acidophilus and Bacillus subtilis) on nitrogen utilization and nutrient digestibility in weaned piglets. A total of 180 piglets (28 days old; initial weight: 8.21 ± 0.67 kg) were allocated to three groups: control (basal diet), FAM (basal diet + 0.1% FAM), and antibiotic (basal diet + 55 mg/kg kitasamycin + 75 mg/kg chlortetracycline). After a 30-day trial, FAM supplementation significantly increased apparent nutrient digestibility of crude protein and ether extract, enhanced duodenal and jejunal digestive enzyme activities, and reduced fecal nitrogen excretion and serum urea nitrogen levels (p < 0.05). Serum metabolomics revealed that FAM upregulated metabolites linked to energy metabolism (e.g., creatine, L-carnosine), which are metabolites of amino acid metabolism, and enriched pathways such as amino acid biosynthesis and protein digestion. These findings demonstrate that FAM improves nitrogen utilization efficiency and gut health in piglets via biosynthesis and metabolism of amino acids, offering a viable alternative to antibiotics.

Recent experiments and simulations have sparked growing interest in the study of Rayleigh–Bénard convection in very slender cells. One pivotal inquiry arising from this interest is the elucidation of the flow structure within these very slender cells. Here we employ tomographic particle image velocimetry, for the first time, to capture experimentally the full-field three-dimensional and three-component velocity field in a very slender cylindrical cell with aspect ratio $\Gamma =1/10$. The experiments cover a Rayleigh number range $5.0 \times 10^8 \leqslant Ra \leqslant 5.0 \times 10^9$ and Prandtl number 5.7. Our experiments reveal that the flow structure in the $\Gamma =1/10$ cell is neither in the multiple-roll form nor in the simple helical form; instead, the ascending and descending flows can intersect and cross each other, resulting in the crossing events. These crossing events separate the flow into segments; within each segment, the ascending and descending flows ascend or descend side by side vertically or in the twisting manner, and the twisting is not unidirectional, while the segments near the boundary can also be in the form of a donut like structure. By applying the mode decomposition analyses to the measured three-dimensional velocity fields, we identified the crossing events as well as the twisting events for each instantaneous flow field. Statistical analysis of the modes reveals that as $Ra$ increases, the average length of the segments becomes smaller, and the average number of segments increases from 2.5 to 3.9 in the $Ra$ range of our experiments.

When implementing Markov Chain Monte Carlo (MCMC) algorithms, perturbation caused by numerical errors is sometimes inevitable. This paper studies how the perturbation of MCMC affects the convergence speed and approximation accuracy. Our results show that when the original Markov chain converges to stationarity fast enough and the perturbed transition kernel is a good approximation to the original transition kernel, the corresponding perturbed sampler has fast convergence speed and high approximation accuracy as well. Our convergence analysis is conducted under either the Wasserstein metric or the $\chi^2$ metric, both are widely used in the literature. The results can be extended to obtain non-asymptotic error bounds for MCMC estimators. We demonstrate how to apply our convergence and approximation results to the analysis of specific sampling algorithms, including Random walk Metropolis, Metropolis adjusted Langevin algorithm with perturbed target densities, and parallel tempering Monte Carlo with perturbed densities. Finally, we present some simple numerical examples to verify our theoretical claims.

Artificial sweeteners are generally used and recommended to alternate added sugar for health promotion. However, the health effects of artificial sweeteners remain unclear. In this study, we included 6371 participants from the National Health and Nutrition Examination Survey with artificial sweetener intake records. Logistic regression and Cox regression were applied to explore the associations between artificial sweeteners and risks of cardiometabolic disorders and mortality. Mendelian randomisation was performed to verify the causal associations. We observed that participants with higher consumption of artificial sweeteners were more likely to be female and older and have above medium socio-economic status. After multivariable adjustment, frequent consumers presented the OR (95 % CI) for hypertension (1·52 (1·29, 1·80)), hypercholesterolaemia (1·28 (1·10, 1·50)), diabetes (3·74 (3·06, 4·57)), obesity (1·52 (1·29, 1·80)), congestive heart failure (1·89 (1·35, 2·62)) and heart attack (1·51 (1·10, 2·04)). Mendelian randomisation confirmed the increased risks of hypertension and type 2 diabetes. Moreover, an increased risk of diabetic mortality was identified in participants who had artificial sweeteners ≥ 1 daily (HR = 2·62 (1·46, 4·69), P = 0·001). Higher consumption of artificial sweeteners is associated with increased risks of cardiometabolic disorders and diabetic mortality. These results suggest that using artificial sweeteners as sugar substitutes may not be beneficial.

Depression is highly prevalent in haemodialysis patients, and diet might play an important role. Therefore, we conducted this cross-sectional study to determine the association between dietary fatty acids (FA) consumption and the prevalence of depression in maintenance haemodialysis (MHD) patients. Dietary intake was assessed using a validated FFQ between December 2021 and January 2022. The daily intake of dietary FA was categorised into three groups, and the lowest tertile was used as the reference category. Depression was assessed using the Patient Health Questionnaire-9. Logistic regression and restricted cubic spline (RCS) models were applied to assess the relationship between dietary FA intake and the prevalence of depression. As a result, after adjustment for potential confounders, a higher intake of total FA [odds ratio (OR)T3 vs. T1 = 1·59, 95 % confidence interval (CI) = 1·04, 2·46] and saturated fatty acids (SFA) (ORT3 vs. T1 = 1·83, 95 % CI = 1·19, 2·84) was associated with a higher prevalence of depressive symptoms. Significant positive linear trends were also observed (P < 0·05) except for SFA intake. Similarly, the prevalence of depression in MHD patients increased by 20% (OR = 1.20, 95% CI = 1.01–1.43) for each standard deviation increment in SFA intake. RCS analysis indicated an inverse U-shaped correlation between SFA and depression (Pnonlinear > 0·05). Additionally, the sensitivity analysis produced similar results. Furthermore, no statistically significant association was observed in the subgroup analysis with significant interaction. In conclusion, higher total dietary FA and SFA were positively associated with depressive symptoms among MHD patients. These findings inform future research exploring potential mechanism underlying the association between dietary FA and depressive symptoms in MHD patients.

To date, a comprehensive understanding of the influence of the Prandtl number ($Pr$) on flow topology in turbulent Rayleigh–Bénard convection (RBC) remains elusive. In this study, we present an experimental investigation into the evolution of flow topology in quasi-two-dimensional turbulent RBC with $7.0 \leq Pr \leq 244.2$ and $2.03\times 10^{8} \leq Ra \leq 2.81\times 10^{9}$. Particle image velocimetry (PIV) measurements reveal the flow transitions from multiple-roll state to single-roll state with increasing $Ra$, and the transition is hindered with increasing $Pr$, i.e. the transitional Rayleigh number $Ra_t$ increases with $Pr$. We mapped out a phase diagram on the flow topology change on $Ra$ and $Pr$, and identified the scaling of $Ra_t$ on $Pr$: $Ra_t \sim Pr^{0.93}$ in the low $Pr$ range, and $Ra_t \sim Pr^{3.3}$ in the high $Pr$ range. The scaling in the low $Pr$ range is consistent with the model of balance of energy dissipation time and plume travel time that we proposed in our previous study, while the scaling in the high $Pr$ range implies a new governing mechanism. For the first time, the scaling of $Re$ on $Ra$ and $Pr$ is acquired through full-field PIV velocity measurement, $Re \sim Ra^{0.63}\,Pr^{-0.87}$. We also propose that increasing horizontal velocity promotes the formation of the large-scale circulation (LSC), especially for the high $Pr$ case. Our proposal was verified by achieving LSC through introducing horizontal driving force $Ra_H$ by tilting the convection cell with a small angle.

This paper retrospectively analysed the prevalence of macrolide-resistant Mycoplasma pneumoniae (MRMP) in some parts of China. Between January 2013 and December 2019, we collected 4,145 respiratory samples, including pharyngeal swabs and alveolar lavage fluid. The highest PCR-positive rate of M. pneumoniae was 74.5% in Beijing, the highest resistance rate was 100% in Shanghai, and Gansu was the lowest with 20%. The highest PCR-positive rate of M. pneumoniae was 74.5% in 2013, and the highest MRMP was 97.4% in 2019; the PCR-positive rate of M. pneumoniae for adults in Beijing was 17.9% and the MRMP was 10.48%. Among the children diagnosed with community-acquired pneumonia (CAP), the PCR-positive and macrolide-resistant rates of M. pneumoniae were both higher in the severe ones. A2063G in domain V of 23S rRNA was the major macrolide-resistant mutation, accounting for more than 90%. The MIC values of all MRMP to erythromycin and azithromycin were ≥ 64 μg/ml, and the MICs of tetracycline and levofloxacin were ≤ 0.5 μg/ml and ≤ 1 μg/ml, respectively. The macrolide resistance varied in different regions and years. Among inpatients, the macrolide-resistant rate was higher in severe pneumonia. A2063G was the common mutation, and we found no resistance to tetracycline and levofloxacin.

Mythimna separata (Lepidoptera: Noctuidae) is an omnivorous pest that poses a great threat to food security. Insect antimicrobial peptides (AMPs) are small peptides that are important effector molecules of innate immunity. Here, we investigated the role of the AMP cecropin B in the growth, development, and immunity of M. separata. The gene encoding M. separata cecropin B (MscecropinB) was cloned. The expression of MscecropinB was determined in different developmental stages and tissues of M. separata. It was highest in the prepupal stage, followed by the pupal stage. Among larval stages, the highest expression was observed in the fourth instar. Tissue expression analysis of fourth instar larvae showed that MscecropinB was highly expressed in the fat body and haemolymph. An increase in population density led to upregulation of MscecropinB expression. MscecropinB expression was also upregulated by the infection of third and fourth instar M. separata with Beauveria bassiana or Bacillus thuringiensis (Bt). RNA interference (RNAi) targeting MscecropinB inhibited the emergence rate and fecundity of M. separata, and resulted in an increased sensitivity to B. bassiana and Bt. The mortality of M. separata larvae was significantly higher in pathogen plus RNAi-treated M. separata than in controls treated with pathogens only. Our findings indicate that MscecropinB functions in the eclosion and fecundity of M. separata and plays an important role in resistance to infection by B. bassiana and Bt.

We report an experimental study about the effect of an obstructed centre on heat transport and flow reversal by inserting an adiabatic cylinder at the centre of a quasi-two-dimensional Rayleigh–Bénard convection cell. The experiments are carried out in a Rayleigh number ($Ra$) range of $2\times 10^7 \leq Ra \leq 2\times 10^9$ and at a Prandtl number ($Pr$) of $5.7$. It is found that for low $Ra$, the obstructed centre leads to a heat transfer enhancement of up to 21 $\%$, while as $Ra$ increases, the magnitude of the heat transfer enhancement decreases and the heat transfer efficiency ($Nu$) eventually converges to that of the unobstructed normal cell. Particle image velocimetry measurements show that the heat transfer enhancement originates from the change in flow topology due to the presence of the cylindrical obstruction. In the low-$Ra$ regime the presence of the obstruction promotes the transition of the flow topology from the four-roll state to the abnormal single-roll state then to the normal single-roll state with increasing obstruction size. While in the high-$Ra$ regime, the flow is always in the single-roll state regardless of the obstruction size, although the flow becomes more coherent with the size of the obstruction. We also found that in the presence of the cylindrical obstruction, the stability of the corner vortices is significantly reduced, leading to a large reduction in the frequency of flow reversals.

SLAM Benchmark plays a pivotal role in the field by providing a common ground for performance evaluation. In this paper, a novel methodology of simultaneous localization and mapping benchmark and map accuracy improvement (SLAMB&MAI) is introduced. It can objectively evaluate errors of localization and mapping, and further improve map accuracy by utilizing evaluation results as feedback. The proposed benchmark transforms all elements into a global frame and measures the errors between them. The comprehensiveness consists in the benchmark of both localization and mapping, and the objectivity consists in the consideration of the correlation between localization and mapping by the preservation of the original pose relations between all reference frames. The map accuracy improvement is realized by first obtaining the optimization that minimizes the errors between the estimated trajectory and ground truth trajectory and then applying it to the estimated map. The experimental results showed that the map accuracy can be improved by an average of 15%. The optimization that yields minimal localization errors is obtained by the proposed Centre Point Registration-Iterative Closest Point (CPR-ICP). This proposed Iterative Closest Point (ICP) variant pre-aligns two point clouds by their centroids and least square planes and then uses traditional ICP to minimize the error between them. The experimental results showed that CPR-ICP outperformed traditional ICP, especially in cases involving large-scale environments. To the extent of our knowledge, this is the first work that can not only objectively benchmark both localization and mapping but also revise the estimated map and increase its accuracy, which provides insights into the acquisition of ground truth map and robot navigation.

Schistosomiasis, a parasite infectious disease caused by Schistosoma japonicum, often leads to egg granuloma and fibrosis due to the inflammatory reaction triggered by egg antigens released in the host liver. This study focuses on the role of the egg antigens CP1412 protein of S. japonicum (SjCP1412) with RNase activity in promoting liver fibrosis. In this study, the recombinant egg ribonuclease SjCP1412, which had RNase activity, was successfully prepared. By analysing the serum of the population, it has been proven that the anti-SjCP1412 IgG in the serum of patients with advanced schistosomiasis was moderately correlated with liver fibrosis, and SjCP1412 may be an important antigen associated with liver fibrosis in schistosomiasis. In vitro, the rSjCP1412 protein induced the human liver cancer cell line Hep G2 and liver sinusoidal endothelial cells apoptosis and necrosis and the release of proinflammatory damage-associated molecular patterns (DAMPs). In mice infected with schistosomes, rSjCP1412 immunization or antibody neutralization of SjCP1412 activity significantly reduced cell apoptosis and necroptosis in liver tissue, thereby reducing inflammation and liver fibrosis. In summary, the SjCP1412 protein plays a crucial role in promoting liver fibrosis during schistosomiasis through mediating the liver cells apoptosis and necroptosis to release DAMPs inducing an inflammatory reaction. Blocking SjCP1412 activity could inhibit its proapoptotic and necrotic effects and alleviate hepatic fibrosis. These findings suggest that SjCP1412 may be served as a promising drug target for managing liver fibrosis in schistosomiasis japonica.

Although a growing body of literature on star employees has focused on top performers, the influence of moral stars has been neglected, an unfortunate situation given that employees’ moral behavior has prolonged impacts on organizations and society as a whole. In this case, we propose the concept of the moral star, defined as the employee (not the team leader) who exhibits disproportionately high and prolonged morality relative to others and has a reputation of being moral on his or her team. We further draw upon self-categorization theory and investigate the double-edged sword effect of the presence of a moral star on the prosocial behavior of other team members. Specifically, we propose that for nonstar employees who have high levels of moral identity, the presence of a moral star is positively related to their felt moral responsibility and prosocial behavior. In contrast, for nonstar employees with low levels of moral identity, the presence of a moral star is negatively related to their felt moral responsibility and prosocial behavior. We found support for our hypotheses across an experiment and a multi-wave and multi-source field study. Taken together, our findings call for closer attention to the recognition of moral stars, as well as their potential unintended negative impact on teams and organizations.

Kazal-type serine protease inhibitors (KaSPI) play important roles in insect growth, development, digestion, metabolism and immune defence. In this study, based on the transcriptome of Mythimna separata, the cDNA sequence of MsKaSPI with Kazal domain was uploaded to GenBank (MN931651). Spatial and temporal expression analysis showed that MsKaSPI was expressed at different developmental stages and different tissues, and it was induced by 20-hydroxyecdysone in third-instar larvae of M. separata. After 24 h infection by Beauveria bassiana, the expression level of MsKaSPI and the corresponding MsKaSPI content were significantly up-regulated, being 6.42-fold and 1.91-fold to the control group, respectively, while the activities of serine protease, trypsin and chymotrypsin were inhibited. After RNA interference interfered with MsKaSPI for 6 h, the expression decreased by 73.44%, the corresponding content of MsKaSPI protein decreased by 55.66% after 12 h, and the activities of serine protease and trypsin were significantly enhanced. Meanwhile, both the larval and pupal stages of M. separata were prolonged, the weights were reduced and the number of eggs per female decreased by 181. Beauveria bassiana infection also increased the mortality of MsKaSPI-silenced M. separata by 18.96%. These prove MsKaSPI can not only result in slow growth and low fecundity of M. separata by regulating the activity of related protease, but also participate in the resistance to pathogenic fungi by regulating the serine protease inhibitor content and the activities of related serine protease.

Extensive evidence has demonstrated that bilinguals non-selectively activate lexicons of both languages when reading or hearing words in one language. Here, we further investigated the electrophysiological roles of cross-linguistic orthography and phonology in the processing of L2 spoken words in unbalanced Chinese (L1)–English (L2) bilinguals in a cross-modal situation. Relative to unrelated control, the recognition of auditory L2 words showed behavioral interference effects when paired with orthographic or phonological neighbors of the correct translations of L2 words. Moreover, the lexical effects were also exhibited in the electrophysiological data, as reflected by marginally less positive late positive component (500–800 ms) amplitudes in the frontal region. Importantly, the orthographic rather than phonological translation neighbor condition elicited less negative N400 (300–500 ms) amplitudes in the parietal–occipital regions, suggesting that this orthographic translation neighbor condition facilitated the co-activation of spoken L2 words. Taken together, these findings indicate that cross-linguistic orthographic and phonological activation have different temporal dynamics with both bottom-up parallel cross-linguistic activation and the top-down inhibitory control mechanism governing the two-language lexical organization in L2 spoken word recognition.

Parallel manipulators are increasingly utilized in extensive industrial applications due to their high accuracy, compact structure, and significant stiffness characteristics. However, most of the time, massive actuators are involved in constructing and controlling a parallel manipulator, which burdens the structure design and controller development. In this paper, a novel underactuated positioning system been built by different sets of linear motion units (defined as the positioning lines) is proposed, enabling to actuate the multiple degree-of-freedom manipulators with one motor. To achieve this, a smart shape memory alloy (SMA) clutch is presented to obtain the positioning function of each positioning line. Further, to get the decoupled motion regulation of the positioning lines, a new thermal kinematic model of the SMA clutch, which considers the heat dissipation influence on the metal components, was built and validated by the physical prototypes. The experimental results show that the constitutive model of the SMA clutch developed in this paper can be validated within the error of 5.3%. It can also be found that the heat dissipation of the metal component has a significant influence on the model accuracy of the SMA clutch (i.e., 2.6% of the model accuracy). The experiments on the underactuated positioning system produce the following results: the single positioning line can achieve high positioning (i.e., average error: 1.01%) and tracking (i.e., average error $\leq$1 mm) abilities; the underactuated positioning system can perform decoupled motions in the three positioning lines with high accuracy (i.e., ±2 mm within the stroke of 180 mm).

This study investigates the dynamics of low-viscosity nanodroplets impacting surfaces with static contact angles from θ = 73° to 180° via molecular dynamics (MD) simulations. Two typical morphologies of impacting nanodroplets are observed at the maximum spreading state, a Hertz-ball-like in a low-Weber-number range and a thin-film-like in a high-Weber-number range. Only inertial and capillary forces dominate the impact for the former, whereas viscous force also becomes dominant for the latter. Regardless of morphologies at the maximum spreading state, the ratio of spreading time to contact time always remains constant on an ideal superhydrophobic surface with θ = 180°. With the help of different kinematic approximations of the spreading time and scaling laws of the contact time, scaling laws of the maximum spreading factor ${\beta _{max}}\sim W{e^{1/5}}$ in the low-Weber-number range (capillary regime) and ${\beta _{max}}\sim W{e^{2/3}}R{e^{ - 1/3}}$ (or ${\beta _{max}}\sim W{e^{1/2}}O{h^{1/3}}$) in the high-Weber-number range (cross-over regime) are obtained. Here, We, Re, and Oh are the Weber number, Reynolds number, and Ohnesorge number, respectively. Although the scaling laws are proposed only for the ideal superhydrophobic surface, they are tested valid for θ over 73° owing to the ignorable zero-velocity spreading effect. Furthermore, combining the two scaling laws leads to an impact number, $W{e^{3/10}}O{h^{1/3}} = 2.1$. This impact number can be used to determine whether viscous force is ignorable for impacting nanodroplets, thereby distinguishing the capillary regime from the cross-over regime.

We present an experimental study of the large-scale vortex (or large-scale circulation, LSC) in turbulent Rayleigh–Bénard convection in a $\varGamma =\text {diameter}/\text {height}=2$ cylindrical cell. The working fluid is deionized water with Prandtl number ($Pr$) around 5.7, and the Rayleigh number ($Ra$) ranges from $7.64\times 10^7$ to $6.06\times 10^8$. We measured the velocity field in various vertical cross-sectional planes by using the planar particle image velocimetry technique. The velocity measurement in the LSC central plane shows that the flow is in the single-roll form, and the centre of the single-roll (vortex) does not always stay at the centre of the cell; instead, it orbits periodically in the direction opposite to the flow direction of the LSC, with its trajectory in the shape of an ellipse. The velocity measurements in the three vertical planes in parallel to the LSC central plane indicate that the flow is in the vortex tube form horizontally filling almost the whole cell, and the centre line of the vortex tube is consistent with the so-called ‘jump rope’ form proposed by a previous study that combined numerical simulation and local velocity measurements in the low $Pr$ case (Vogt et al., Proc. Natl Acad. Sci. USA, vol. 115, 2018, pp. 12674–12679). In addition, we found that the oscillation of the local velocity in $\varGamma =2$ cells originates from the periodical orbiting of the vortex centre. Our velocity measurements further indicate that the vortex centre orbiting is absent in $\varGamma =1$ cells, at least in the $Ra$ range of our experiments.