Green water loads on prismatic obstacles (representing topside structures) mounted on the raised deck of a simplified vessel are investigated using computational fluid dynamics simulations and physical model testing with emphasis on examining different structure shapes, orientation angles and relative structure size. For each scenario investigated, several flow features are identified that characterize the green water interaction with the structure and influence loads, namely delayed flow diversion, formation of a vertical jet, scattered wave formation and the development of complex wake patterns. Comparing across structures, these interactions are more pronounced for blunt objects, and the associated force impulse is larger. For example, a cube with flow at normal incidence is found to experience approximately twice the force impulse of a circular cylinder of the same projected area. Equally, rotation of the cube leads to reduced run-up height and streamwise force on the structure. To explain these trends, a theoretical model based on Newtonian flow theory is adopted. This model provides an estimate of the streamwise force exerted on obstacles in high-Froude-number flows and shows good agreement with the numerical results when the flow is supercritical, shallow (small water depth relative to structure width) and the structure is tall (large structure height relative to water depth). Despite some limitations, the model should provide an efficient force prediction tool for practical use in design.

In this paper, on–off switching digitization of a W-band variable gain power amplifier (VGPA) with above 60 dB dynamic range is introduced for large-scale phased array. Digitization techniques of on–off switching modified stacking transistors with partition are proposed to optimize configuration of control sub-cells. By the proposed techniques, gain control of a radio frequency variable gain amplifier (VGA) could be highly customized for both coarse and fine switching requirements instead of using additional digital-to-analog converters to tune the overall amplifier bias. The designed VGA in 130 nm SiGe has achieved switchable gain range from −46.4 to 20.6 dB and power range from −25.0 to 15.7 dBm at W band. The chip size of the fabricated VGPA is about 0.31 mm × 0.1 mm.

An investigation into an outbreak of Salmonella Newport infections in Canada was initiated in July 2020. Cases were identified across several provinces through whole-genome sequencing (WGS). Exposure data were gathered through case interviews. Traceback investigations were conducted using receipts, invoices, import documentation, and menus. A total of 515 cases were identified in seven provinces, related by 0–6 whole-genome multi-locus sequence typing (wgMLST) allele differences. The median age of cases was 40 (range 1–100), 54% were female, 19% were hospitalized, and three deaths were reported. Forty-eight location-specific case sub-clusters were identified in restaurants, grocery stores, and congregate living facilities. Of the 414 cases with exposure information available, 71% (295) had reported eating onions the week prior to becoming ill, and 80% of those cases who reported eating onions, reported red onion specifically. The traceback investigation identified red onions from Grower A in California, USA, as the likely source of the outbreak, and the first of many food recall warnings was issued on 30 July 2020. Salmonella was not detected in any tested food or environmental samples. This paper summarizes the collaborative efforts undertaken to investigate and control the largest Salmonella outbreak in Canada in over 20 years.

Previous work suggests that the arrangement of elements in an obstruction may influence the bulk flow velocity through the obstruction, but the physical mechanisms for this influence are not yet clear. This is the motivation for this study, where direct numerical simulation is used to investigate flow through an array of cylinders at a resolution sufficient to observe interactions between wakes of individual elements. The arrangement is altered by varying the gap ratio $G/d$ (1.2 – 18, G is the distance between two adjacent cylinders, d is the cylinder diameter), array-to-element diameter ratio $D/d$ (3.6 – 200, D is the array diameter), and incident flow angle ($0^{\circ} - 30^{\circ}$). Depending on the element arrangement, it is found that the average root-mean-square lift and drag coefficients can vary by an order of magnitude, whilst the average time-mean drag coefficient of individual cylinders ($\overline{C_{d}}$), and the bulk velocity are found to vary by up to $50\,\%$ and a factor of 2, respectively. These arrangement effects are a consequence of the variation in flow and drag characteristics of individual cylinders within the array. The arrangement effects become most critical in the intermediate range of flow blockage parameter $\mathit{\Gamma_{D}^{\prime}} = 0.5-1.5$ ($\mathit{\Gamma_{D}^{\prime}}=\overline{C_{d}}aD/(1-\phi)$, where a is frontal element area per unit volume, and $\phi$ is solid volume fraction), due to the high variability in element-scale flow characteristics. Across the full range of arrangements modelled, it is confirmed that the bulk velocity is governed by flow blockage parameter but only if the drag coefficient incorporates arrangement effects. Using these results, this paper proposes a framework for describing and predicting flow through an array across a variety of arrangements.

China is still among the 30 high-burden tuberculosis (TB) countries in the world. Few studies have described the spatial epidemiological characteristics of pulmonary TB (PTB) in Jiangsu Province. The registered incidence data of PTB patients in 95 counties of Jiangsu Province from 2011 to 2021 were collected from the Tuberculosis Management Information System. Three-dimensional spatial trends, spatial autocorrelation, and spatial–temporal scan analysis were conducted to explore the spatial clustering pattern of PTB. From 2011 to 2021, a total of 347,495 newly diagnosed PTB cases were registered. The registered incidence rate of PTB decreased from 49.78/100,000 in 2011 to 26.49/100,000 in 2021, exhibiting a steady downward trend (χ2 = 414.22, P < 0.001). The average annual registered incidence rate of PTB was higher in the central and northern regions. Moran’s I indices of the registered incidence of PTB were all >0 (P< 0.05) except in 2016, indicating a positive spatial correlation overall. Local autocorrelation analysis showed that ‘high–high’ clusters were mainly distributed in northern Jiangsu, and ‘low–low’ clusters were mainly concentrated in southern Jiangsu. The results of this study assist in identifying settings and locations of high TB risk and inform policy-making for PTB control and prevention.

Although there is a range of approaches for classifying the wake structure behind an array of obstacles, these approaches provide inconsistent results across different array systems. This motivates the present study to integrate and reconcile these approaches into one that is consistent across different systems. This new, transferable classification approach is based on the dimensionless flow blockage of the array and the wake stability parameter. To demonstrate this approach, a series of laboratory experiments was conducted to characterise the wake structure behind an array of emergent cylinders across a practically relevant parameter space that has not previously been explored. Two arrays with the same values of flow blockage and wake stability parameters but different sizes display the same wake structure, demonstrating the controlling influence of these two parameters on the wake structure. This approach classifies four different wake structures, which are distinct in that they display differences in instantaneous and time-averaged flow fields, temporal velocity oscillations, shear layer growth and the length of the steady wake region. The dependence of the wake structure on the two parameters is a consequence of (i) the controlling influence of blockage on the fraction of incident flow passing through the array and (ii) the ability of shallowness to suppress wake instabilities and, to a lesser extent, also influence the velocity through the array. This paper provides a predictive framework for the wake structure based on knowledge of the array geometry, and the depth and velocity of incident flow across the entire relevant practical parameter space.

Nontuberculous mycobacteria (NTM) is a large group of mycobacteria other than the Mycobacterium tuberculosis complex and Mycobacterium leprae. Epidemiological investigations have found that the incidence of NTM infections is increasing in China, and it is naturally resistant to many antibiotics. Therefore, studies of NTM species in clinical isolates are useful for understanding the epidemiology of NTM infections. The present study aimed to investigate the incidence of NTM infections and types of NTM species. Of the 420 samples collected, 285 were positive for M. tuberculosis, 62 samples were negative, and the remaining 73 samples contained NTM, including 35 (8.3%) only NTM and 38 (9%) mixed (M. tuberculosis and NTM). The most prevalent NTM species were Mycobacterium intracellulare (30.1%), followed by Mycobacterium abscessus (15%) and M. triviale (12%). M. gordonae infection was detected in 9.5% of total NTM-positive cases. Moreover, this study reports the presence of Mycobacterium nonchromogenicum infection and a high prevalence of M. triviale for the first time in Henan. M. intracellulare is the most prevalent, accompanied by some emerging NTM species, including M. nonchromogenicum and a high prevalence of M. triviale in Henan Province. Monitoring NTM transmission and epidemiology could enhance mycobacteriosis management in future.

The flow-induced vibrations (FIVs) of two identical tandem square cylinders with mass ratio m* = 3.5 at Reynolds number Re = 150 are investigated through two-dimensional direct numerical simulation (DNS) and linear stability analysis over a parameter range of spacing ratio 1.5 ≤ L* ≤ 5 and reduced velocity 3 ≤ Ur ≤ 34. Three kinds of FIV responses, namely vortex-induced vibration (VIV), biased oscillation (BO) and galloping (GA), are identified. The FIVs are then further classified into the branches of initial VIV (IV), resonant VIV (RV and RV′), flutter-induced VIV (FV), desynchronized VIV (DV), VIV developing from GA (GV), transitional state between VIV and GA (TR), BO and GA based on the characteristics of the vibration responses. The transitions among different FIV branches are examined by combining the DNS with linear stability analysis, where the transition boundaries among the VIV, BO and GA branches over the concerned parameters are identified on the branch maps. The transition from IV to RV or RV′ is found to be related to the unstable wake mode, while the FV, transiting from RV or RV′, is induced by the unstable structural factor in the wake-structure mode. The structural instability is considered as the physical origin of GA, whereas the mode competition between unstable wake and structure leads to DV, GV and TR, and thus delays the appearance of GA. The transition from DV to BO with biased equilibrium position, accompanied by the even-order harmonic frequencies, is essentially induced by the symmetry breaking bifurcation.

Three-dimensional (3-D) wake transitions of a steady flow past two side-by-side circular cylinders are investigated through Floquet analysis and direct numerical simulations (DNS) over the gap-to-diameter ratio $g^*$ up to 3.5 and Reynolds number ${\textit {Re}}$ up to 400. When the flows behind two cylinders form in-phase and anti-phase wakes at large $g^*$, the wake transition is similar to the isolated cylinder counterpart, with the critical ${\textit {Re}}$ for the onset of 3-D transition (${\textit {Re}}_{cr-1}$) happens at around 180. At small $g^*$, 3-D transition becomes interestingly complex due to the distinct characteristics formed in base flows. The ${\textit {Re}}_{cr-1}$ suddenly drops to around 60–100 and forms distinct variation trends with $g^*$. Precisely, the ${\textit {Re}}_{cr-1}$ of the single symmetric wake (SS, $g^*\lessapprox 0.25$) is more than half of the isolated cylinder counterpart due to the large length scale of the SS wake. Only mode A is detected in SS. In the asymmetric single wake (ASS, $g^* \approx 0.25\unicode{x2013}0.6$) and flip-flop wake (FF, $g^* \approx 0.6\unicode{x2013}1.8$), the 3-D transition develops at ${\textit {Re}} \approx 103\unicode{x2013}60$ and 75–60, respectively. The decrease in ${\textit {Re}}_{cr-1}$ with increasing $g^*$ is because of the increased level of wake asymmetry in ASS and irregular vortex shedding in FF. Floquet analysis predicts two new unstable modes, namely mode A$'$ and subharmonic mode C$'$, of ASS. Both modes are transient features in 3-D DNS and the flow eventually saturates into a new 3-D mode, mode ASS. The gap flow of mode ASS is distinctly characterised by its time-independent spanwise waviness structure that is deflected towards different transverse directions with a long wavelength of about $14$ cylinder diameters. The 3-D mode of the FF is irregular both temporally and spatially. Variations of ${\textit {Re}}_{cr-1}$ with $g^*$, the characteristics and the physical mechanisms of each 3-D mode are discussed in this study.

An emergent volume electron microscopy technique called cryogenic serial plasma focused ion beam milling scanning electron microscopy (pFIB/SEM) can decipher complex biological structures by building a three-dimensional picture of biological samples at mesoscale resolution. This is achieved by collecting consecutive SEM images after successive rounds of FIB milling that expose a new surface after each milling step. Due to instrumental limitations, some image processing is necessary before 3D visualization and analysis of the data is possible. SEM images are affected by noise, drift, and charging effects, that can make precise 3D reconstruction of biological features difficult. This article presents Okapi-EM, an open-source napari plugin developed to process and analyze cryogenic serial pFIB/SEM images. Okapi-EM enables automated image registration of slices, evaluation of image quality metrics specific to pFIB-SEM imaging, and mitigation of charging artifacts. Implementation of Okapi-EM within the napari framework ensures that the tools are both user- and developer-friendly, through provision of a graphical user interface and access to Python programming.

The impact of Strouhal number St (= 0.1–1.0), Reynolds number Re (= 50–2000) and dimensionless wavelength λ (= 0.5–2.0) on the hydrodynamic performance of a travelling wavy foil of a constant length is extensively investigated. The relationship of time-mean thrust with St, Re and λ is presented, suggesting that the propulsive force increases with increasing St, Re and λ. As such, the drag–thrust boundary advances as these parameters increase. A shorter λ makes the thrust steadier while a longer λ enhances the maximum instantaneous thrust. The latter is beneficial for prey to escape from a predator. The fluid added mass caused by the foil oscillation increases with St and λ but declines with Re (<500). Seven types of wake structures produced by the foil are identified, discussed and connected to thrust generation, showing how St, Re and λ affect the fluid dynamics, wake transition, vortex strength, wake jet, velocity, added mass, added damping, power input, efficiency and pressure profiles. The outcome of this work renders a physical basis for understanding the swimming of aquatic animals.

The turbulent kinetic energy and energy dissipation rate in the wake of a circular cylinder are examined at a Reynolds number of 1000. The turbulence characteristics are quantified using direct numerical simulation, which provides a comprehensive dataset that is almost impossible to acquire from physical experiments. The energy dissipation rate is decomposed into the components due to the mean flow, the coherent primary vortices and the remainder. It is found that the remainder component, which develops only in a three-dimensional turbulent wake and resides mainly in the regions of vortices, accounts for 95 % and 97 % of the total dissipation rate for 10 and 20 cylinder diameters downstream of the cylinder, respectively (while the remainder accounts for 62 % and 83 % of the total turbulent kinetic energy). Based on the remainder component, the validity of local isotropy, local axisymmetry, local homogeneity and homogeneity in the y–z plane for the turbulent dissipation in the wake is examined. The analysis reveals that the turbulent dissipation is largely locally homogeneous, but not locally isotropic or axisymmetric, even after the annihilation of the primary vortex street. In addition, the performances of the four corresponding surrogates to the true dissipation rate are evaluated. Owing to the general validity of local homogeneity, the surrogates of local homogeneity and homogeneity in the y–z plane perform well. Although local axisymmetry does not hold, the corresponding surrogate performs well, because errors from different terms largely cancel out. However, the surrogate of local isotropy generally underestimates the true dissipation rate.

The essence of sub-critical transition of oscillatory boundary-layer flows is the non-modal growth of finite-amplitude disturbances. The current understanding of the mechanisms of the orderly and bypass transitions of oscillatory boundary-layer flows is limited. The present study adopts optimisation approaches to predict the maximum energy amplification of two- and three-dimensional perturbations in response to the optimal initial disturbance with or without external forcing. A series of direct numerical simulations are also performed to compare with the results obtained from the stability analyses. In particular, the optimal initial perturbation similar to a Tollmien–Schlichting (T–S) wave yields the largest transient growth under the combined effects of the Orr mechanism and inflectional point instability. With a considerable level of two-dimensional disturbance, the vortex tube nonlinearly develops from the T–S-like wave, and then either deforms into a $\varLambda$-vortex in the near-wall region or rolls up to the free shear region. The further burst of turbulence can follow the first pathway as K-type transition or the second one as vortex tube breakdown due to the elliptical instability. Additionally, non-modal growth can initiate the inception of streaky structures by favourable three-dimensional initial perturbations and/or forcing. The secondary instabilities responsible for the streak breakdown are classified as the varicose (symmetric) and sinuous (anti-symmetric) modes. Under a sufficiently high level of three-dimensional disturbance, the bypass transition is predominantly characterised by the formation of the sinuous mode and turbulent spots, which leads to the suppression of inflection point instability.