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4 results

  • Chapter 13 - Set-Up Procedures for Optimizing Performance in the IVF Laboratory

  • from Section 2 - Pre-procedure Protocols
  • Edited by Markus H. M. Montag, ilabcomm GmbH, St Augustin, Germany, Dean E. Morbeck, Kindbody Inc, New York City
  • Book:
    Principles of IVF Laboratory Practice
    Published online:
    07 August 2023
    Print publication:
    24 August 2023, pp 96-104

  • Summary

    Optimization of procedures routinely performed in a clinical human in vitro fertilization (IVF) laboratory have become increasingly important due to the increase in complexity of procedures now performed in the laboratory. The addition of new technologies requiring more oversight has increased dramatically within the last decade. As a result of incorporating these new technologies, safe and efficient operation of the IVF laboratory has become increasingly complex and requires a substantial understanding of processes within the laboratory. In today’s modern IVF laboratory, the amount of staff time to perform every increasingly complicated case has more than doubled. Similarly, the amount required time to prepare for these cases has increased dramatically as well. In many instances, the increase in complexity of laboratory procedures has not translated into hiring of new staff but the creation of challenges to improve efficiency within the laboratory. The current guidelines for allocation of staff are based upon cycle numbers performed on an annual basis, not complexity of cases performed.

  • Chapter 26 - The beneficial effects of culture medium supplementation with growth factors in the development of human embryos in vitro

  • from Section 5 - Laboratory
  • Edited by Gab Kovacs, Monash University, Victoria
  • Book:
    How to Improve your ART Success Rates
    Published online:
    05 July 2011
    Print publication:
    30 June 2011, pp 143-146

  • Summary

    During in vivo development, maternally and embryonic-derived growth factors play a role in preimplantation development. The addition of growth factors to culture medium for human pre-implantation development has had beneficial effects on embryo development and quality. Examples of some of the growth factors that have proven to have beneficial effects are: granulocyte macrophage colony stimulating-factor (GM-CSF), epidermal growth factor (EGF), insulin-like growth factors (IGF), leukemia inhibitory factor (LIF), platelet activating factor (PAF), and platelet-derived growth fact (PDGF). Culture of human embryos in the presence of GM-CSF has shown increased rates of blastocyst formation as well as total number of cells allocated to the inner cell mass (ICM) and trophectoderm. A factor that is unique in terms of clinical application in assisted reproduction is hyaluronan. The culture media formulations in use today contain the necessary substrates in order to support the development of competent blastocysts.