We present the first results from the COS-EDGES survey, targeting the kinematic connection between the interstellar medium and multi-phase circumgalactic medium (CGM) in nine isolated, near-edge-on galaxies at $z\sim0.2$, each probed along its major axis by a background quasar at impact parameters of $D=13-38$ kpc. Using VLT/UVES and HST/COS quasar spectra, we analyse Mgi, Mgii, Hi, Cii, Ciii, and Ovi absorption relative to galaxy rotation curves from Keck/LRIS and Magellan/MagE spectra. We find that low ionisation absorption for 8/9 galaxies lies below the halo escape velocity, indicating bound inflow or recycling gas, while 6/9 galaxies have high ionisation gas reaching above the halo escape velocity, suggesting some unbound material. We find that at lower $D/R_{\textrm{vir}}$ ($0.12\leq D/R_{\textrm{vir}} \leq0.20$), over 80% of absorption in all ions lies on the side of systemic velocity matching disk rotation, and the optical-depth–weighted median velocity ($v_{abs}$) is consistent with the peak rotation speed. At higher $D/R_{\textrm{vir}}$ ($0.21\leq D/R_{\textrm{vir}} \leq0.31$), the kinematics diverge by ionisation state: For the low ionisation gas, the amount of co-rotating absorption remains above 80%, yet $v_{abs}$ drops to roughly 60% of the galaxy rotation speed. For the high ionisation gas (Ovi), only 60% of the absorption is consistent with co-rotation and $v_{abs}$ drops to 20% of the galaxy rotation speed. Furthermore, the velocity widths, corresponding to 50% of the total optical depth ($\Delta v_{50}$) for low ionisation gas is up to 1.8 times larger in the inner halo than at larger radii, while for Ciii and Ovi $\Delta v_{50}$ remains unchanged with distance. The 90% optical-depth width ($\Delta v_{90}$) shows a modest decline with radius for low ionisation gas but remains constant Ciii and Ovi. At high $D/{R}_{\textrm{vir}}$, both $\Delta v_{50}$ and $\Delta v_{90}$ increase with ionisation potential. These results suggest a radially dependent CGM kinematic structure: the inner halo hosts cool, dynamically broad gas tightly coupled to disk rotation, whereas beyond $\gtrsim 0.2 R_{\textrm{vir}}$, particularly traced by Ovi and Hi, the CGM shows weaker rotational alignment and lower relative velocity dispersion. Therefore, low-ionisation gas likely traces extended co-rotating gas, inflows and/or recycled accretion, while high-ionisation gas reflects a mixture of co-rotating, lagging, discrete collisionally ionised structures and volume-filling warm halo, indicating a complex kinematic stratification of the multi-phase CGM.

Current evidence underscores a need to transform how we do clinical research, shifting from academic-driven priorities to co-led community partnership focused programs, accessible and relevant career pathway programs that expand opportunities for career development, and design of trainings and practices to develop cultural competence among research teams. Failures of equitable research translation contribute to health disparities. Drivers of this failed translation include lack of diversity in both researchers and participants, lack of alignment between research institutions and the communities they serve, and lack of attention to structural sources of inequity and drivers of mistrust for science and research. The Duke University Research Equity and Diversity Initiative (READI) is a program designed to better align clinical research programs with community health priorities through community engagement. Organized around three specific aims, READI-supported programs targeting increased workforce diversity, workforce training in community engagement and cultural competence, inclusive research engagement principles, and development of trustworthy partnerships.

Objectives/Goals: Cerebral amyloid angiopathy (CAA) characterized by the accumulation of amyloid-beta in the cerebrovasculature, affects blood vessel integrity leading to brain hemorrhages and an accelerated cognitive decline in Alzheimer’s disease patients. In this study, we are conducting a genome-wide association study to identify genetic risk factors for CAA. Methods/Study Population: We genotyped 1253 additional AD cases using and curated existing genome-wide genotype data from 110 AD and 502 non-AD donors from the Mayo Clinic Brain Bank. We performed QC and imputation of all datasets. We conducted GWAS in AD only (N =  1,363), non-AD only, as well as the combined cohort (N = 1,865) by testing imputed variant dosages for association with square root transformed CAA using linear regression, adjusting for relevant covariates. To assess associations in the context of major CAA risk factors, we performed interaction analysis with APOEe4 presence and sex; and pursued stratified analyses. We collected peripheral gene expression measures using RNA isolated from 188 PAXgene tube samples of 95 donors collected across multiple time points. More than 1/3 of these participants have MRI measures collected. Results/Anticipated Results: Variants at the APOE locus were identified as the most significant in our study. In addition, several other variants with suggestive association were found under the main model adjusting for AD neuropathology (Braak and Thal). LINC-PINT splice variant remained associated with lower CAA scores in AD cases without the APOEe4 risk allele. To enhance the robustness of our findings, we are pursuing further expansion of our study cohort. In the periphery, we expect to identify expression changes associated with neuroimaging indicators of CAA and determine if variants and genes discovered via GWAS are implicated in these changes. Discussion/Significance of Impact: We expect this study will provide further insight into the genetic architecture underlying risk for CAA both in the context of significant AD pathology and without. Characterization of genetic variants and functional outcomes in the context of neuropathology may lead to new avenues of research aimed at identifying biomarkers and therapies to treat CAA

According to International Union for the Conservation of Nature (IUCN) guidelines, all species must be assessed against all criteria during the Red Listing process. For organismal groups that are diverse and understudied, assessors face considerable challenges in assembling evidence due to difficulty in applying definitions of key terms used in the guidelines. Challenges also arise because of uncertainty in population sizes (Criteria A, C, D) and distributions (Criteria A2/3/4c, B). Lichens, which are often small, difficult to identify, or overlooked during biodiversity inventories, are one such group for which specific difficulties arise in applying Red List criteria. Here, we offer approaches and examples that address challenges in completing Red List assessments for lichens in a rapidly changing arena of data availability and analysis strategies. While assessors still contend with far from perfect information about individual species, we propose practical solutions for completing robust assessments given the currently available knowledge of individual lichen life-histories.

This study examined the predictors and sequelae of exposure to peer pressure from close friends in adolescence. Adolescents (99 female; 85 male) were followed from age 13 to 24 utilizing peer, parent, and romantic partner reports and observational data. Participants who were exposed to high levels of peer pressure as teens were more likely to experience higher levels of coercive behavior from romantic partners (as reported by those partners), as well as lower levels of parent-reported functional independence. All findings held even after accounting for baseline levels of teen assertiveness. Adolescents at risk for increasing exposure to peer pressure were characterized by poor-quality parent and peer relationships, as well as baseline deficits in ability to assert autonomy. Results suggest that exposure to peer pressure, aside from its potential effects on deviant or risky behavior, may reflect a powerful threat to the autonomy development process as adolescents transition from parents to peers as primary sources of support and interaction.

Jellyfishes have ecological and societal value, but our understanding of taxonomic identity of many jellyfish species remains limited. Here, an approach integrating morphological and molecular (16S ribosomal RNA and cytochrome oxidase I) data enables taxonomic assessment of the blubber jellyfish found in the Philippines. In this study, we aimed to resolve doubt on the taxonomy of Acromitoides purpurus, a valid binomen at the time of our research. Our morphological findings confirm that this jellyfish belongs to the genus Catostylus, and is distinct from known species of the genus inhabiting the Western Pacific, such as Catostylus ouwensi, Catostylus townsendi, and Catostylus mosaicus. Detailed morphological and molecular analyses of the type specimens from the Philippines with the other Catostylus species revive the binomen Catostylus purpurus and invalidate A. purpurus. Genetic analysis also distinguishes this Philippine jellyfish from C. townsendi and C. mosaicus. Through this study, we arranged several Catostylidae taxa into species inquirendae (Catostylus tripterus, Catostylus turgescens, and Acromitoides stiphropterus) and one genus inquirenda (Acromitoides) and provided an identification key for species of Catostylus. This comprehensive study confirms the blubber jellyfish as C. purpurus, enriching our understanding of jellyfish biodiversity. The integration of morphological and genetic analyses proves vital in resolving taxonomic ambiguities within the Catostylidae family and in the accurate identification of scyphozoan jellyfishes.

The ways that psychopathology manifests in adolescence have shifted dramatically over the past twenty-five years, with rates of many externalizing behaviors declining substantially while rates of anxiety and depressive disorders have skyrocketed. This paper argues that understanding these changes requires rethinking the field’s historically somewhat negative views of intense peer connections, peer influences, and adolescent risk-taking behavior. It is argued that intense peer connections are critical to development, and that peer influence and risk taking have important, often overlooked, adaptive components. The shift in observed manifestations of adolescent psychopathology over this period can be viewed at least partly in terms of a shift away from strong peer connections and toward greater risk aversion. Implications for research and intervention based on a focus on the adaptive aspects of peer influences and risk taking are discussed.

Evidence from previous research suggests that frame-of-reference (FOR) training is effective at improving assessor ratings in many organizational settings. Yet no research has presented a thorough examination of systematic sources of variance (assessor-related effects, evaluation settings, and measurement design features) that might influence training effectiveness. Using a factorial ANOVA and variance components analyses on a database of four studies of frame-of-reference assessor training, we found that (a) training is most effective at identifying low levels of performance and (b) the setting of the training makes little difference with respect to training effectiveness. We also show evidence of the importance of rater training as a key determinant of the quality of performance ratings in general. Implications for FOR training theory and practice are discussed.

This 20-year prospective study examined verbal aggression and intense conflict within the family of origin and between adolescents and their close friends as predictors of future verbal aggression in adult romantic relationships. A diverse community sample of 154 individuals was assessed repeatedly from age 13 to 34 years using self-, parent, peer, and romantic partner reports. As hypothesized, verbal aggression in adult romantic relationships was best predicted by both paternal verbal aggression toward mothers and by intense conflict within adolescent close friendships, with each factor contributing unique variance to explaining adult romantic verbal aggression. These factors also interacted, such that paternal verbal aggression was predictive of future romantic verbal aggression only in the context of co-occurring intense conflict between an adolescent and their closest friend. Predictions remained robust even after accounting for levels of parental abusive behavior toward the adolescent, levels of physical violence between parents, and the overall quality of the adolescent’s close friendship. Results indicate the critical importance of exposure to aggression and conflict within key horizontal relationships in adolescence. Implications for early identification of risk as well as for potential preventive interventions are discussed.

This 19-year prospective study applied a social development lens to the challenge of identifying long-term predictors of adult negative affectivity. A diverse community sample of 169 individuals was repeatedly assessed from age 13 to age 32 using self-, parent-, and peer-reports. As hypothesized, lack of competence establishing and maintaining close friendships in adolescence had a substantial long-term predictive relation to negative affectivity at ages 27–32, even after accounting for prior depressive, anxious, and externalizing symptoms. Predictions also remained robust after accounting for concurrent levels of depressive symptoms, indicating that findings were not simply an artifact of previously established links between relationship quality and depressive symptoms. Predictions also emerged from poor peer relationships within young adulthood to future relative increases in negative affectivity by ages 27–32. Implications for early identification of risk as well as for potential preventive interventions are discussed.

This article examines the critical reception of Feist's 2017 album Pleasure, and interpretations by reviewers of the mysterious hiss that permeates most of the album's tracks. I firstly contextualise Pleasure in relation to the aesthetics of record production. I then examine interviews with Feist and her collaborators to identify the source of the hiss, and explain its presence on Pleasure. Lastly, I examine a corpus of 20 reviews, showcasing how critics (mis)identified and (mis)interpreted the sound, as well as the effect of this on their overall understanding of the album. To explain the relationship between the hiss and accompanying music, I assume a semiotic perspective. Following Poyatos, I regard these two kinds of sound as part of distinct yet related cosystems of signs, loosely analogous to the relationship between verbal utterances and nonverbal behaviours in face-to-face communication. Through this lens, I analyse how Pleasure's hiss was heard to modify, support, emphasise and undermine the meaning of the music by reviewers.

This 17-year prospective study applied a social-development lens to the challenge of identifying long-term predictors of adult depressive symptoms. A diverse community sample of 171 individuals was repeatedly assessed from age 13 to age 30 using self-, parent-, and peer-report methods. As hypothesized, competence in establishing close friendships beginning in adolescence had a substantial long-term predictive relation to adult depressive symptoms at ages 27–30, even after accounting for prior depressive, anxiety, and externalizing symptoms. Intervening relationship difficulties at ages 23–26 were identified as part of pathways to depressive symptoms in the late twenties. Somewhat distinct paths by gender were also identified, but in all cases were consistent with an overall role of relationship difficulties in predicting long-term depressive symptoms. Implications both for early identification of risk as well as for potential preventive interventions are discussed.

The thirty-four-year reign of President Porfirio Díaz (1876–1910) is generally acknowledged to have been the period of Mexico's great economic transformation. In reality, Mexico was unable to avoid the accelerated change that overtook it during the last quarter of the nineteenth century, as proliferating patterns of trade, which accompanied the burgeoning industrial development of the United States and Western Europe, tied Mexico ever more closely to the global economy. An international economic division of labor was negotiated between foreign industrialists and entrepreneurs—who urgently needed primary products, markets for goods, and opportunities for investment, and national and regional elites—who welcomed infrastructural improvements, modern machinery, an array of consumer goods, and the increasing availability of foreign capital.