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Building a successful research career often requires being adept at the methods and tools of the time. For social and behavioral scientists today, that means navigating online participant platforms and the tools used to create online studies. In this chapter, we describe how Amazon’s Mechanical Turk (MTurk) can be leveraged as a source for participant recruitment. We provide a brief history of MTurk’s usage by researchers, describe the challenges researchers have faced with the site, and summarize the status of issues like data quality, sample representativeness, and ethics in online research. Along the way, we provide tips for how researchers can use MTurk to collect high-quality data and to start and advance a research career.
We examined how relative language dominance impacts Spanish–English bilinguals’ crosslinguistic and nonlinguistic interference resolution abilities during a web-based Spanish picture-word interference naming task and a subsequent spatial Stroop paradigm, and the relationship between the two. Results show the expected interference and facilitation effects in the online setting across both tasks. Additionally, participants with greater English dominance had larger within-language, Spanish facilitation and marginally larger crosslinguistic (English to Spanish) interference effects reflected on accuracy performance. Similarly, participants with greater English dominance had larger nonlinguistic congruency facilitation effects. Our results are in line with other studies finding a relation between linguistic and nonlinguistic cognitive control. Correlated reaction time performance between the linguistic and nonlinguistic paradigms suggests that overcoming crosslinguistic interference may be partly based on cognitive control processes used outside of language. Modulations by language dominance underline the importance of accounting for relative language proficiency in bilinguals’ two languages when studying bilingualism.
The purpose of this paper is to examine the contours of evolving jurisprudence on offensive expression and negative messages, and to suggest that it can best be understood by reference to the concept of stigma. At the European Court of Human Rights, there appears to have been an increasing willingness to recognise the harm of offensive expression through an interpretation of Article 8 of the European Convention on Human Rights, but the reach of this case law remains uncertain. In particular, while some cases associate negative expression with negative stereotyping, not all of these cases do, and there are potential conflicts with freedom of speech. In the domestic context, these issues recently arose in a significant case from the Court of Appeal, R (Crowter) v Secretary of State for Health and Social Care. In this case, the appellants argued that a legal provision sends a negative ‘message’, through the negative stereotyping of disabled people, but this ‘message’ is implicit, rather than explicitly articulated. While these developments raise important questions about the future evolution of case law, we propose that a focus on stigma can more clearly highlight the harms involved.
Southern Sudan has a low population density, abundant land and tremendous agricultural potential. A large number of domesticated crops are grown in the region in a range of cropping systems. There are also numerous useful wild plant genetic resources. Little collecting work has been done in the region, there are few accessions from southern Sudan stored ex situ and publications on the actual and potential plant genetic resources for agriculture are sparse. The region has been a centre of civil conflict with little respite since independence in 1956. The farmers’ fields and natural environments represent in situ genebanks, which following the cessation of hostilities will become extremely important for the rehabilitation of subsistence agriculture, the promotion of cash crop production and the revitalization of the regional economy. Several wild plant species are highly nutritious and merit the attention of plant scientists. This article describes some of the domesticated, semi-domesticated and wild plant genetic resources of the area, and suggests why these are important for agricultural rehabilitation following implementation of a peace accord.
This article explores the effects of naming and describing disability in law and medicine. Instead of focusing on substantive issues like medical treatment or legal rights, it will address questions which arise in relation to the use of language itself. When a label which is attached to a disability is associated with a negative meaning, this can have a profound effect on the individual concerned and can create stigma. Overly negative descriptions of disabilities can be misleading, not only for the individual, but also more broadly in society, if there are inaccurate perceptions about disability in the social context. This article will examine some relevant examples of terminology, where these issues arise. It will also suggest that the role of medicine and the law in naming and describing disability is particularly important because in these areas there is, perhaps more than anywhere else, a recognized source of authority for the choice of terminology. Labels and descriptions used in the medical and legal contexts can not only perpetuate existing stigmatization of disabled people, but can also contribute to creating stigma at its source, given that the words used in these contexts can constitute an exercise of power.
Infection prevention program leaders report frequent use of criteria to distinguish recently recovered coronavirus disease 2019 (COVID-19) cases from actively infectious cases when incidentally positive asymptomatic patients were identified on routine severe acute respiratory coronavirus virus 2 (SARS-CoV-2) polymerase chain reaction (PCR) testing. Guidance on appropriate interpretation of high-sensitivity molecular tests can prevent harm from unnecessary precautions that delay admission and impede medical care.
Cancer has been associated with lower risk of dementia, although methodological issues raise concerns about the validity of this association. We recruited 31,080 men aged 65–85 years who were free of cancer and dementia, and followed them for up to 22 years. We used health record linkage to identify incident cases of cancer and dementia, and split time span to investigate this association. 18,693 (60.1%) and 6897 (22.2%) participants developed cancer and dementia during follow-up. The hazard ratio (HR) of dementia associated with cancer was 1.13 (95% CI = 1.07, 1.20) and dropped to 0.85 (95% CI = 0.80, 0.91) when 449 participants who developed dementia within 2 years were excluded. The diagnosis of cancer seems to facilitate the early detection of dementia cases. Older participants who survive cancer for 2 or more years have lower risk of receiving the diagnosis of dementia over time. The factors that mediate this association remain unclear.
There is evidence that the COVID-19 pandemic has negatively affected mental health, but most studies have been conducted in the general population.
Aims
To identify factors associated with mental health during the COVID-19 pandemic in individuals with pre-existing mental illness.
Method
Participants (N = 2869, 78% women, ages 18–94 years) from a UK cohort (the National Centre for Mental Health) with a history of mental illness completed a cross-sectional online survey in June to August 2020. Mental health assessments were the GAD-7 (anxiety), PHQ-9 (depression) and WHO-5 (well-being) questionnaires, and a self-report question on whether their mental health had changed during the pandemic. Regressions examined associations between mental health outcomes and hypothesised risk factors. Secondary analyses examined associations between specific mental health diagnoses and mental health.
Results
A total of 60% of participants reported that mental health had worsened during the pandemic. Younger age, difficulty accessing mental health services, low income, income affected by COVID-19, worry about COVID-19, reduced sleep and increased alcohol/drug use were associated with increased depression and anxiety symptoms and reduced well-being. Feeling socially supported by friends/family/services was associated with better mental health and well-being. Participants with a history of anxiety, depression, post-traumatic stress disorder or eating disorder were more likely to report that mental health had worsened during the pandemic than individuals without a history of these diagnoses.
Conclusions
We identified factors associated with worse mental health during the COVID-19 pandemic in individuals with pre-existing mental illness, in addition to specific groups potentially at elevated risk of poor mental health during the pandemic.
The aim of this study is to systematically investigate the demographic and disease predictors of cognitive and behavioural phenotype in the largest cohort of children with NF1 published to date. Based on previously published research, we examine the potential role of demographic predictors such as age, sex, SES, parental NF1 status as well as the neurological complications such as epilepsy and brain tumours in NF1 associated cognitive/ behavioural impairments.
Method
In this cross-sectional study design, participant data were drawn from two large databases which included (i) A clinical database of all patients with NF1 seen in a clinical psychological service from 2010 to 2019 and (ii) A research dataset from two previously published studies (2,8). The complex National NF1 service based within Manchester regional genetic services is set up for individuals with complex NF1 (https://www.mangen.co.uk/healthcare-professionals/clinical-genomic-services/nf1/) in the North of the UK. Children were referred to the psychological services by NF1 clinicians if psychological assessment was warranted based on parental reports. In order to reduce clinic referral bias, the clinical sample was supplemented by including participants that were seen solely for the purposes of research studies within our centre.
Result
Relative to population norms, 90% of the NF1 sample demonstrated significantly lower scores in at least one cognitive or behavioral domain. Family history of NF1 and lower SES were independently associated with poorer cognitive, behavioral and academic outcomes. Neurological problems such as epilepsy and hydrocephalus were associated with lower IQ and academic skills.
Conclusion
Cognitive and behavioural phenotypes commonly emerge via a complex interplay between genes and environmental factors, and this is true also of a monogenic condition such as NF1. Early interventions and remedial education may be targeted to risk groups such those with familial NF1, families with lower SES and those with associated neurological comorbidities.
To identify a posteriori dietary patterns among women planning pregnancy and assess the reproducibility of these patterns in a subsample using two dietary assessment methods.
Design:
A semi-quantitative FFQ was administered to women enrolled in the Singapore PREconception Study of long-Term maternal and child Outcomes study. Dietary patterns from the FFQ were identified using exploratory factor analysis (EFA). In a subsample of women (n 289), 3-d food diaries (3DFD) were also completed and analysed. Reproducibility of the identified patterns was assessed using confirmatory factor analysis (CFA) in the subsample, and goodness of fit of the CFA models was examined using several fit indices. Subsequently, EFA was conducted in the subsample and dietary patterns of the FFQ and the 3DFD were compared.
Setting:
Singapore.
Participants:
1007 women planning pregnancy (18–45 years).
Results:
Three dietary patterns were identified from the FFQ: the ‘Fish, Poultry/Meat and Noodles’ pattern was characterised by higher intakes of fish, poultry/meat and noodles in soup; ‘Fast Food and Sweetened Beverages’ pattern was characterised by higher intakes of fast food, sweetened beverages and fried snacks; ‘Bread, Legumes and Dairy’ pattern was characterised by higher intakes of buns/ethnic breads, nuts/legumes and dairy products. The comparative fit indices from the CFA models were 0·79 and 0·34 for the FFQ and 3DFD of the subsample, respectively. In the subsample, three similar patterns were identified in the FFQ while only two for the 3DFD.
Conclusions:
Dietary patterns from the FFQ are reproducible within this cohort, providing a basis for future investigations on diet and health outcomes.
Implementation of genome-scale sequencing in clinical care has significant challenges: the technology is highly dimensional with many kinds of potential results, results interpretation and delivery require expertise and coordination across multiple medical specialties, clinical utility may be uncertain, and there may be broader familial or societal implications beyond the individual participant. Transdisciplinary consortia and collaborative team science are well poised to address these challenges. However, understanding the complex web of organizational, institutional, physical, environmental, technologic, and other political and societal factors that influence the effectiveness of consortia is understudied. We describe our experience working in the Clinical Sequencing Evidence-Generating Research (CSER) consortium, a multi-institutional translational genomics consortium.
Methods:
A key aspect of the CSER consortium was the juxtaposition of site-specific measures with the need to identify consensus measures related to clinical utility and to create a core set of harmonized measures. During this harmonization process, we sought to minimize participant burden, accommodate project-specific choices, and use validated measures that allow data sharing.
Results:
Identifying platforms to ensure swift communication between teams and management of materials and data were essential to our harmonization efforts. Funding agencies can help consortia by clarifying key study design elements across projects during the proposal preparation phase and by providing a framework for data sharing data across participating projects.
Conclusions:
In summary, time and resources must be devoted to developing and implementing collaborative practices as preparatory work at the beginning of project timelines to improve the effectiveness of research consortia.
We present a study of optical and electronic properties of solutions and films based on the fungi-derived pigment xylindein, extracted from decaying wood and processed without and with a simple purification step (“ethanol wash”). The “post-wash” xylindein solutions exhibited considerably lower absorption in the ultraviolet spectral range and dramatically reduced photoluminescence below 600 nm, due to removal of contaminants most likely to be fungal secondary metabolites. The “post-wash” xylindein-based films were characterized by two orders of magnitude higher charge carrier mobilities as compared to “pre-wash” samples. This underlines the importance of minimizing contaminants that disrupt the conductive xylindein network in xylindein-based electronic devices.
Determining bilingual status has been complicated by varying interpretations of what it means to be bilingual and how to quantify bilingual experience. We examined multiple indices of language dominance (self-reported proficiency, self-reported exposure, expressive language knowledge, receptive language knowledge, and a hybrid), and whether these profiles related to performance on linguistic and cognitive tasks. Participants were administered receptive and expressive vocabulary tasks in English and Spanish, and a nonlinguistic spatial Stroop task. Analyses revealed a relation between dominance profiles and cognate and nonlinguistic Stroop effects, with somewhat different patterns emerging across measures of language dominance and variable type (continuous, categorical). Only a hybrid definition of language dominance accounted for cognate effects in the dominant language, as well as nonlinguistic spatial Stroop effects. Findings suggest that nuanced effects, such as cross-linguistic cognate effects in a dominant language and cognitive control abilities, may be particularly sensitive to operational definitions of language status.
We present on the optical and electronic properties of a fungi-derived pigment xylindein for potential use in (opto)electronic applications. Optical absorption spectra in solutions of various concentrations and in film are compared and are consistent with aggregate formation in concentrated solutions and films. In order to improve film morphology obtained by solution deposition techniques, an amorphous polymer PMMA was introduced to xylindein to form xylindein:PMMA blends. Current-voltage characteristics and hole mobilities extracted from space-charge limited currents were found to be comparable between pristine xylindein and xylindein:PMMA films. Side by side comparison of the photoresponse of pristine xylindein and xylindein:PMMA films at 633 nm revealed an increase in the photosensitivity in xylindein:PMMA films due to the improved morphology favouring enhanced charge generation.
'Silver Xtra Sweet’, ‘How Sweet It Is', ‘Zenith’ and 'Sweetie 76’ were evaluated for response to 0, 35, and 70 g/ha nicosulfuron applied POST at the 5- to 6- or 7- to 8-leaf stages of growth. Nicosulfuron (35 g/ha) caused 60 to 80% visible injury to Silver Xtra Sweet. How Sweet It Is and Zenith were moderately tolerant (< 20% visible injury) and Sweetie 76 was the most tolerant (10% visible injury). All cultivars were less tolerant when nicosulfuron was applied at the 7- to 8-leaf than the 5- to 6-leaf stage. Plant height of Silver Xtra Sweet was reduced 51% by 35 g/ha nicosulfuron applied at the 5- to 6-leaf stage and injury increased when nicosulfuron was applied at the 7- to 8-leaf stage. Yield losses of Silver Xtra Sweet occurred as a result of the nicosulfuron treatments.
In 1991 and 1993, tolerance of ‘Zenith’ and ‘Merit’ sweet corn to 35 g ai/ha nicosulfuron post-directed (PDIR) 0, 5, 10, and 15 cm up the corn stalk or applied POST was determined. In 1991, nicosulfuron applied POST to Zenith caused approximately 30% visible injury, 30% height reduction, and 50% reduction of U.S. No. 1 marketable ear weight. In 1991 no visible injury was observed in the PDIR treatments. Zenith was not injured by any treatment in 1993. Both years, Merit was killed by nicosulfuron applied POST. In 1991 and 1993, nicosulfuron PDIR 0 and 5 cm up the corn stalk of Merit caused approximately 5% and 65% visible injury, respectively, and resulted in yield loss. PDIR application increased nicosulfuron tolerance of moderately tolerant Zenith but did not improve that of least tolerant Merit.
A search of recent political science literature and conference presentations shows substantial fascination with the concept of the natural experiment. However, there seems to be a wide array of definitions and applications employed in research that purports to analyze natural experiments. In this introductory essay to the special issue, we attempt to define natural experiments and discuss related issues of research design. In addition, we briefly explore the basic methodological issues around the appropriate analysis of natural experiments and give an overview of different techniques. The overarching theme of this essay and of this issue is to encourage applied researchers to look for natural experiments in their own work and to think more systematically about research design.