Maladaptive daydreaming is a distinct syndrome in which the main symptom is excessive vivid fantasising that causes clinically significant distress and functional impairment in academic, vocational and social domains. Unlike normal daydreaming, maladaptive daydreaming is persistent, compulsive and detrimental to one’s life. It involves detachment from reality in favour of intense emotional engagement with alternative realities and often includes specific features such as psychomotor stereotypies (e.g. pacing in circles, jumping or shaking one’s hands), mouthing dialogues, facial gestures or enacting fantasy events. Comorbidity is common, but existing disorders do not account for the phenomenology of the symptoms. Whereas non-specific therapy is ineffective, targeted treatment seems promising. Thus, we propose that maladaptive daydreaming be considered a formal syndrome in psychiatric taxonomies, positioned within the dissociative disorders category. Maladaptive daydreaming satisfactorily meets criteria for conceptualisation as a psychiatric syndrome, including reliable discrimination from other disorders and solid interrater agreement. It involves significant dissociative aspects, such as disconnection from perception, behaviour and sense of self, and has some commonalities with but is not subsumed under existing dissociative disorders. Formal recognition of maladaptive daydreaming as a dissociative disorder will encourage awareness of a growing problem and spur theoretical, research and clinical developments.

The anthropogenic spread of disease from captive to wild amphibian populations (referred to as spillover) is linked to global amphibian declines. Disinfecting procedures and protocols exist to mitigate pathogen transmission to and within natural areas, but understanding of visitor attitudes and behaviour regarding their adoption is limited. We surveyed visitors in two natural areas in a global amphibian biodiversity hotspot to assess their attitudes regarding pathogen spread in such areas and analysed the factors influencing their behavioural intentions to take specific actions to prevent pathogen spillover. Visitors’ willingness to take action was influenced by their attitudes, behavioural control and trust in wildlife/land managers, whereas socio-demographic characteristics were less influential. These findings help us to understand visitor behaviour with respect to amphibian biosecurity in natural areas and inform enhanced biosecurity measures and strategic messaging to reduce pathogen spillover.

This work investigated the effects of repeated sweet taste exposure at breakfast on perceptions and intakes of other sweet foods, while also examining the effects due to duration of exposure (1/3 weeks), test context (breakfast/lunch) and associations between taste perceptions and intakes. Using a randomised controlled parallel-group design, participants (n 54, 18 male, mean age: 23·9 (sd 5·8) years, mean BMI: 23·6 (sd 3·5) kg/m2) were randomised to consume either a sweet breakfast (cereal with sucralose) (n 27) or an equienergetic non-sweet breakfast (plain cereal) (n 27) for 3 weeks. On days 0 (baseline), 7 and 21, pleasantness, desire to eat and sweetness were rated for other sweet and non-sweet foods and sweet food consumption was assessed in an ad libitum meal at breakfast and lunch. Using intention-to-treat analyses, no statistically significant effects of exposure were found at breakfast (largest F2,104 = 1·84, P = 0·17, ηp2 = 0·03) or lunch (largest F1,52 = 1·22, P = 0·27, ηp2 = 0·02), and using Bayesian analyses, the evidence for an absence of effect in all rating measures was strong to very strong (smallest BF01 = 297·97 (BF01error = 2·68 %)). Associations between ratings of pleasantness, desire to eat and intake were found (smallest r = 0·137, P < 0·01). Effects over time regardless of exposure were also found: sugars and percentage energy consumed from sweet foods increased throughout the study (smallest (F2,104 = 4·54, P = 0·01, ηp2 = 0·08). These findings demonstrate no effects of sweet taste exposure at breakfast for 1 or 3 weeks on pleasantness, desire for, sweetness or intakes of other sweet foods in either the same (breakfast) or in a different (lunch) meal context.

This work investigated the photophysical pathways for light absorption, charge generation, and charge separation in donor–acceptor nanoparticle blends of poly(3-hexylthiophene) and indene-C60-bisadduct. Optical modeling combined with steady-state and time-resolved optoelectronic characterization revealed that the nanoparticle blends experience a photocurrent limited to 60% of a bulk solution mixture. This discrepancy resulted from imperfect free charge generation inside the nanoparticles. High-resolution transmission electron microscopy and chemically resolved X-ray mapping showed that enhanced miscibility of materials did improve the donor–acceptor blending at the center of the nanoparticles; however, a residual shell of almost pure donor still restricted energy generation from these nanoparticles.

Implementation of a novel experimental approach using a bright source of narrowband x-ray emission has enabled the production of a photoionized argon plasma of relevance to astrophysical modelling codes such as Cloudy. We present results showing that the photoionization parameter ζ = 4πF/ne generated using the VULCAN laser was ≈ 50 erg cm s−1, higher than those obtained previously with more powerful facilities. Comparison of our argon emission-line spectra in the 4.15 - 4.25 Å range at varying initial gas pressures with predictions from the Cloudy code and a simple time-dependent code are also presented. Finally we briefly discuss how this proof-of-principle experiment may be scaled to larger facilities such as ORION to produce the closest laboratory analogue to a photoionized plasma.

The sorption behaviour of I−, Cs+, Ni2+, Eu3+, Th4+ and UO2+ 2on NRVB (Nirex reference vault backfill) a possible vault backfill, at pH 12.8 was studied. Sorption isotherms generated were compared to results obtained in the presence of cellulose degradation products (CDP). Whereas Cs was not affected by the presence of the organic compounds, a notable reduction in the sorption of Th and Eu to cement was observed. The results also indicated limited removal of Ni from solution (with or without an organic ligand) by sorption, the concentration in solution seemingly being determined solely by solubility processes. In the case of uranium, the presence of CDP increased the sorption to cement by almost one order of magnitude. Further studies into the uptake of CDP by cement are being undertaken to identify the mechanism(s) responsible.

Polymer:fullerene nanoparticles (NPs) offer two key advantages over bulk heterojunction (BHJ) films for organic photovoltaics (OPVs), water-processability and potentially superior morphological control. Once an optimal active layer morphology is reached, maintaining this morphology at OPV operating temperatures is key to the lifetime of a device. Here we study the morphology of the PDPP-TNT (poly{3,6-dithiophene-2-yl-2,5-di(2-octyldodecyl)-pyrrolo[3,4-c]pyrrole-1,4-dione-alt-naphthalene}):PC71BM ([6,6]-phenyl C71 butyric acid methyl ester) NP system and then compare the thermal stability of NP and BHJ films to the common poly(3-hexylthiophene) (P3HT): phenyl C61 butyric acid methyl ester (PC61BM) system. We find that material T g plays a key role in the superior thermal stability of the PDPP-TNT:PC71BM system; whereas for the P3HT:PC61BM system, domain structure is critical.

Unintentional herbicide resistance gene stacking in canola may alter the sensitivity of volunteers to herbicides of alternative modes of action commonly used for their control. Greenhouse experiments were conducted to investigate the response of three single-herbicide–resistant (HR) cultivars (glyphosate, glufosinate, imidazolinone), one non-HR cultivar, and seven multiple (double or triple)–HR experimental lines to 2,4-D (amine and ester), MCPA ester, and metribuzin applied at the two- to three-leaf stage and of one non-HR and four HR cultivars (glyphosate, glufosinate, imidazolinone, bromoxynil) to 2,4-D amine applied at two growth stages (two- to three-leaf stage and five- to six-leaf stage). All canola cultivars or lines treated at the two- to three-leaf stage responded similarly to increasing doses of each of the three herbicides. At the five- to six-leaf stage, however, the bromoxynil HR cultivar was less sensitive to 2,4-D than the other cultivars. The results of this study suggest that canola with multiple-herbicide–resistance traits does not differ from cultivars that are non-HR or single HR in its sensitivity to herbicides commonly used to control volunteers. All volunteers, whether non-HR, single HR, or multiple HR, should be treated when plants are most sensitive to herbicides (two- to four-leaf stage) to reduce their interference against crops and their perpetuation of gene flow.

Cleavers species (false cleavers and catchweed bedstraw) are among the top 10 most abundant weeds across the prairie region of western Canada, and are increasing in relative abundance at the fastest rate since the 1970s. In 2008, two false cleavers populations from Tisdale and Choiceland, Saskatchewan, were suspected of acetolactate synthase (ALS) –inhibitor resistance. Dose-response experiments were conducted with the use of imazethapyr and florasulam, both ALS inhibitors, as well as fluroxypyr, a synthetic auxin. Additionally, a 1,954–base-pair region of the ALS gene including sites known to confer ALS resistance were sequenced. Both populations were highly resistant to imazethapyr (resistance factors greater than 100), one population (Tisdale) was highly resistant to florasulam (Choiceland population susceptible, although a second, larger screening of 200 individuals indicated low frequency [2%] florasulam resistance), and both populations were susceptible to fluroxypyr. All sequenced Tisdale individuals screened with imazethapyr posessed the Trp574Leu mutation. In contrast, three point mutations were found for Choiceland individuals sequenced: Ser653Asn, Trp574Leu, and Asp376Glu. These ALS target-site mutations have not been documented previously in this species.

Multiple cases of ALS inhibitor-resistant weed biotypes are reported for many species, including wild mustard. The physiological extent and molecular basis of resistance to ALS inhibitors was compared in four biotypes of wild mustard from western Canada: a sulfonylurea (SU)-resistant (R) biotype from Manitoba detected in 1992; an SU (ethametsulfuron)-R biotype from Alberta detected in 1993 (metabolism-based resistance); an SU-R biotype from Manitoba detected in 2002; and a SU- and imidazolinone (IMI)-R biotype from Saskatchewan detected in 2002. Herbicide dose-response experiments confirmed that the two Manitoba biotypes were resistant to the SU herbicides ethametsulfuron and tribenuron : thifensulfuron mixture, whereas the Saskatchewan biotype was resistant to both SU herbicides and to imazethapyr, an IMI herbicide. Sequence analysis of the ALS gene detected target site mutations in three of the four R biotypes, with amino acid substitutions Pro197 (CCT) to Ser (TCT) [Domain A of the gene] in the two SU-R Manitoba biotypes and Trp574 (TGG) to Leu (TTG) [Domain B] in the Saskatchewan biotype. The Alberta SU-R biotype had the same ALS nucleotide and amino acid sequence as the susceptible population at these two positions. Two heterozygous individuals [Trp574 (Tt/gG)] were detected in the Saskatchewan biotype, and genetic segregation for nucleotide bases and resistance phenotype was consistent with single gene control. Nucleotide variation in neutral regions of the ALS gene varied with biotype, with no variation in the two Manitoba biotypes, two variants in the Saskatchewan biotype, and 16 neutral nucleotide polymorphisms (0.9%) in the Alberta biotype. The occurrence of at least three different ALS inhibitor-R biotypes in this important weed species is likely to impact negatively on the use of ALS inhibitors, such as the IMIs, and serves as a warning for strict implementation of herbicide rotations to prevent or delay the evolution and spread of such populations.

Over 90% of Canadian kochia populations are resistant to acetolactatesynthase (ALS)– inhibiting herbicides. We questioned whether the targetsite–based resistance could affect plant growth and competitiveness.Homozygous F2 herbicide-resistant (HR) kochia plants with anamino acid substitution at Trp574 (sources: Alberta [AB],Saskatchewan [SK], and Manitoba [MB]), or Pro197 (MB, AB with twopopulations) were grown in replacement series with homozygous F2herbicide-susceptible (HS) plants from the corresponding heterogeneouspopulation (total: six populations). In pure stands, growth of HR plantsfrom AB and SK was similar to that of HS plants, regardless of mutation;conversely, MB2-HR plants (Trp574Leu) developed more slowly andwere taller than MB2-HS plants. Final dry weight of HR plants in pure standswas similar across all six populations, whereas that for HS plants in purestands and HR–HS plants in mixed stands (50–50%) varied with population.Results for AB and SK populations suggest little impact of either ALSmutation on kochia growth, whereas those for MB lines would suggest anunidentified factor (or factors) affecting the HS, HR, or both biotypes. Thevariable response within and between lines, and across HS biotypeshighlights the importance of including populations of various origins andmultiple susceptible controls in HR biotype studies.

Early Holocene sediments from a continental Antarctic lake (Ace Lake, Vestfold Hills, East Antarctica) contained abundant fossil rotifers of the genus Notholca. The fossil is similar to specimens of Notholca sp. present in modern-day Ace Lake and other fresh and brackish lakes of the Vestfold Hills. Cyanobacteria and protists (chrysophyte cysts, dinoflagellate cysts, and rhizopod tests) were also recovered from the core samples. These sediments were deposited early in the freshwater phase of Ace Lake, soon after deglaciation of the area. The occurrence of this trophically diverse assemblage of organisms at an early stage in the evolution of the lake suggests either that they were part of an endemic Antarctic flora and fauna which pre-dated the last glacial maximum and survived in glacial refugia or that efficient intercontinental dispersal had occurred.

A survey of 109 fields was conducted across western Canada in spring 2007 to determine the extent of ALS-inhibitor and dicamba (synthetic auxin) resistance in kochia. Weed seedlings were collected from fields in three provinces of western Canada and transplanted into the greenhouse. Seeds were harvested from selfed plants, and the F1 progeny were screened for resistance to the ALS-inhibitor mixture thifensulfuron–tribenuron or dicamba. All kochia populations were susceptible to dicamba. ALS inhibitor–resistant kochia was found in 85% of the fields surveyed in western Canada: 80 of 95 fields in Alberta, six of seven fields in Saskatchewan, and all seven fields in Manitoba. For the 93 ALS inhibitor–resistant populations, the mean frequency (±SE) of parental plants classified as resistant was 61 ± 3%. Most of the resistant populations (87%) were heterogeneous and contained both resistant and susceptible individuals. ALS sequence data (Pro197 and Asp376 mutations) and genotyping data (Trp574 mutation) obtained for 87 kochia parental (i.e., field-collected) plants confirmed the presence of all three target-site mutations as well as two mutational combinations (Pro197 + Trp574, Asp376 + Trp574) in resistant individuals.

The molecular basis for acetolactate synthase (ALS)–inhibitor resistance was determined for 6 susceptible (HS) and 24 resistant (HR) kochia populations from western Canada. The latter included 3 HR populations from Alberta (AB), 3 from Manitoba (MB), and 18 from Saskatchewan (SK). HR plants survived application of the ALS-inhibitor herbicide thifensulfuron–tribenuron mixture in the greenhouse. Most of the HR populations were heterogeneous and contained both HR and HS individuals. The molecular basis for resistance was determined in 273 HR individuals by sequencing the ALS gene (2,270 base pair [bp]) or by conducting a TaqMan genotyping assay developed in this study using real-time polymerase chain reaction (PCR) for single nucleotide polymorphism (SNP) 1709, where a G to T substitution resulted in a Leu for Trp substitution at amino acid position 574 (Trp574Leu mutation). A total of 16 SNPs were identified in the ALS gene sequences (0.7% polymorphism), 5 of which resulted in amino acid changes that confer resistance to ALS-inhibiting herbicides. The SNPs correspond to three target-site mutations: Pro197 (SNPs 565 and 566), Asp376 (SNP 1116), and Trp574 (SNPs 1708 and 1709). The Trp574Leu mutation was predominant (189 HR plants). The next most common mutation was the highly variable residue Pro197 (44 HR plants) with substitution by one of nine amino acids. The least-frequent were Asp376Glu (9 plants) and Trp574Arg (3 plants) substitutions. The presence of two ALS target-site mutations was found in 30 individual kochia plants, the first report from field-selected weed populations. These include combinations Pro197 + Trp574 (23 plants) and Pro197 + Asp376 (7 plants). The detection of Pro197, Asp376, and Trp574 mutations, as well as both combinations, from geographically separate regions suggests multiple origins of these mutations.

ALS inhibitor-resistant biotypes are the fastest growing class ofherbicide-resistant (HR) weeds. A Canadian ALS inhibitor-resistant biotypeof Russian thistle was first reported in 1989. The molecular basis forALS-inhibitor resistance is unknown for Canadian populations of thispolyploid weed species, and was determined in this study for one Alberta andtwo Saskatchewan HR Russian thistle populations. HR plants survived sprayapplication of the ALS-inhibitor mixture thifensulfuron : tribenuron in thegreenhouse. All three HR Russian thistle populations were heterogeneous andcontained both HR and herbicide-susceptible (HS) individuals. The molecularbasis for resistance was determined by sequencing the ALSgene and/or conducting a TaqMan genotyping assay for single nucleotidepolymorphism (SNP) for the Trp574Leu mutation. Two target-sitemutations were observed: Trp574Leu in all three biotypes (554individuals) and Pro197Gln in one biotype (one individual),suggesting multiple-founding events for Russian thistle HR populations inwestern Canada. Segregation patterns among F1 and F2 progeny arrays of HRlines sprayed under greenhouse conditions varied; some segregated (i.e., hadHR and HS progeny), whereas other lines were exclusively HR. In contrast, nosegregation of molecular types, i.e., Trp574, Trp/Leu574 and Leu574, as would be expected withheterozygosity at a single locus Trp/Leu574, was observed. Suchlack of segregation is consistent with the polyploid genome structure ofRussian thistle and the presence of two copies of the ALSgene. The presence of more than one ALS gene confounded theability of the molecular techniques to accurately identify “true”heterozygotes in this study.

Wild buckwheat is the most abundant broadleaf weed across the Prairie region of western Canada. Acetolactate synthase (ALS)-inhibiting herbicides are commonly used to control this species and other broadleaf weeds in cereal crops. A field survey in Alberta in 2007 identified a single population that was putatively resistant to ALS-inhibiting herbicides. In herbicide resistance screening in the greenhouse, all F1 progeny tested were resistant to the ALS-inhibiting herbicides thifensulfuron/tribenuron, a sulfonylurea herbicide, or florasulam, a triazolopyrimidine herbicide; dose response of shoot biomass indicated the population was 10- and 20-fold less sensitive to thifensulfuron/tribenuron and florasulam, respectively, than a susceptible control population. ALS gene sequencing of 24 F1 progeny indicated that the Trp574Leu target-site mutation was responsible for conferring ALS-inhibitor resistance in this biotype, the first global report of ALS-inhibitor resistance for this species. Because this mutation typically endows high-level resistance across all five ALS-inhibitor classes, this wild buckwheat biotype may only be controlled by a different site-of-action herbicide.