Coconut oil, extracted from coconut kernels, is a rich source of medium-chain fatty acids (MCFAs), including lauric acid, capric acid and caprylic acid. This experiment aimed to investigate the protective effect of coconut oil against intestinal injury induced by lipopolysaccharide (LPS) challenge in piglets. A total of 24 piglets were used in a 2 × 2 factorial experiment with dietary treatment (3% soybean oil vs 3% coconut oil) and LPS challenge (saline vs LPS). After 28 days of the experiment, piglets were injected intraperitoneally with LPS (100 μg/kg body weight) or saline. Piglets were slaughtered and sampled for testing. Pigs fed coconut oil had higher average daily gain and body weight during the entire study. Supplementation with coconut oil improved intestinal morphology and barrier function, indicated by increased jejunal villus height, as well as enhanced protein expression of ZO-1 and Occuldin. Furthermore, coconut oil supplementation improved plasma antioxidant capacity indicated by enhanced glutathione peroxidase (GSH-Px) activity and decreased malondialdehyde (MDA) concentration. Moreover, Coconut oil ameliorated the LPS-induced release of pro-inflammatory cytokines, as indicated by decreased IL-1β expression in the jejunum. Coconut oil also alleviated the up-regulation of the expression of necroptosis protein receptor-interacting protein kinase 3 (RIPK3) and mixed lineage kinase-like protein (MLKL) in the jejunum of piglets stimulated by LPS. In conclusion, dietary supplementation of coconut oil can improve the growth performance of piglets, and alleviate LPS-induced intestinal injury and inflammation by inhibiting necroptosis signaling pathway.

This study evaluated the effect of different medium-chain to long-chain fatty acid (MCFA:LCFA, M:L) ratios on growth performance, intestinal function, antioxidant capacity and gut microbiota in piglets. A total of 250 piglets were randomly assigned to five groups with five replicates, each containing ten pigs. The diets, containing varying amounts of MCFA-rich coconut oil and LCFA-rich soyabean oil, resulted in M:L ratios of 0, 2·1, 4·2, 8·8 and 33·8 %. Results showed that both final body weight and average daily weight gain increased as the M:L ratio increased (P < 0·05), while the 8·8 % M:L ratio diet exhibited the lowest feed:gain ratio (P < 0·05). As the M:L ratio increased, the contents of superoxide dismutase and glutathione peroxidase were increased, and MDA was decreased in serum (P < 0·05). The 8·8 and 33·8 % M:L diets improved ileal and jejunal morphology (P < 0·05), as indicated by greater villus height and villus height:crypt depth ratios. Furthermore, increasing M:L ratios from 0 to 33·8 % increased expression of tight junction proteins occludin and ZO-1 in the jejunum (P < 0·05). The 33·8 % M:L ratio reduced microbial α-diversity (P < 0·05), while 8·8 % M:L diet significantly increased the abundance of beneficial bacteria (e.g. Lactobacilli, Prevotella) and decreased harmful bacteria (e.g. Escherichia-Shigella, Enterococcus) in the cecum (P < 0·05). In summary, our study found that 8·8 % of dietary M:L ratios significantly improved growth performance, likely through modulating intestinal function, antioxidant activity and gut microbial composition.

This paper proposes a highly selective dual-band bandpass filter (BPF) utilizing hemispherical resonators and an in-band transmission zeros (TZs) method. For obtaining high isolation between two passbands, a compound resonator topology is realized by arranging six hemispherical cavity resonators (HCRs) in a two-by-two vertical configuration, and then three TZs between the passbands are introduced. The filter selectivity is further optimized through elaborately placing three metal pillars in the cavity, and then one TZ in the lower stopband and two in the upper stopband are generated. Notably, the size of the HCR is reduced by about 50% compared to the conventional spherical resonator. To validate the design, a dual-band filter is 3D printed, which operates at 9.1 and 9.77 GHz with bandwidths of 210 and 200 MHz, respectively. The measured results show good agreement with the simulated ones.

Pipeline inspection robots play a crucial role in maintaining the integrity of pipeline systems across various industries. In this paper, a novel pipeline inspection robot is designed based on a four degrees-of-freedom (DOF) generalized parallel mechanism (GPM). First, a four DOF mechanism is introduced using numerical and graph synthesis. The design employs numerical and graph synthesis methods to achieve an ideal symmetric configuration, enhancing the robot’s adaptability and mobility. The coupling mid-platform, inspired by parallelogram mechanisms, enables synchronized contraction motion, allowing the robot to adjust to different pipe diameters. Then, the constraints of the pipeline inspection robot in the elbow are analyzed based on task requirements. Through kinematic and performance analyses using screw theory, the mechanism’s feasibility in practical applications is confirmed. Theoretical analysis, simulations, and experiments demonstrate the robot’s ability to achieve active steering in T-branches and elbows. Experimental validation in straight and bent pipes shows that the robot meets the expected speed targets and can successfully navigate complex pipeline environments. This research highlights the potential of GPMs in advancing the capabilities of pipeline inspection robots for real-world applications.

Intestinal health challenges – including dysbiosis, inflammatory disorders, and pathogen susceptibility – impose severe economic losses and welfare concerns in intensive livestock production. Functional lipids, defined as bioactive lipid molecules with physiological benefits beyond basic nutrition, offer promising solutions to these issues. This review establishes a comprehensive definition of functional lipids and elucidates their metabolic process. Using short- and medium-chain fatty acid glycerides as a prime example, we examine their significant roles in energy homeostasis, gut microbiota composition and diversity, immune modulation, and antibacterial and antiviral activities. Additionally, we critically evaluate their current applications and future industrial potential in livestock production, providing evidence-based recommendations for their optimal implementation in animal nutrition strategies.

Cable-driven parallel robots (CDPRs) have been widely used as motion executers for their large workspace and lower inertia. However, there are few studies on structural optimization design considering its stability. This paper proposes a stability optimization method based on force-position workspace for a reconfigurable cable-driven parallel robot (RCDPR). First, the structural optimization analysis of RCDPR is carried out. Then, the forces distribution algorithm based on the feasibility of real-time control is determined, and the boundary contour algorithm (BCA) of the RCDPR force feasible workspace (FFW) on the central plane is proposed. Second, the stiffness and cables driving force space (CFS) models of RCDPR are established. Subsequently, the stability evaluation function is established to optimize the structure of RCDPR, which uses FFW and main task feasible workspace (MFW) as carriers and stiffness and CFS as weights. Finally, an experimental prototype of the developed robot is constructed, and motion performance and workspace verification experiments are conducted. The results demonstrate that the developed RCDPR has good motion accuracy and stable workspace, and the results also verify the feasibility of the stability evaluation function and BCA.

The sulphur microbial diet (SMD), a dietary pattern associated with forty-three sulphur-metabolising bacteria, may influence gut microbiota composition and contribute to ageing process through gut-produced hydrogen sulfide (H2S). We aimed to explore the association between SMD and biological age (BA) acceleration, using the cross-sectional study that included 71 579 individuals from the UK Biobank. The SMD score was calculated by multiplying β-coefficients by corresponding serving sizes and summing them, based on dietary data collected using the Oxford WebQ, a 24-hour dietary assessment tool. BA was assessed using Klemerae–Doubal (KDM) and PhenoAge methods. The difference between BA and chronological age refers to the age acceleration (AgeAccel), termed ‘KDMAccel’ and ‘PhenoAgeAccel’. Generalised linear regression was performed. Mediation analyses were used to investigate underlying mediators including BMI and serum aspartate aminotransferase/alanine aminotransferase (AST/ALT) ratio. Following adjustment for multiple variables, a positive association was observed between consuming a dietary pattern with a higher SMD score and both KDMAccel (βQ4 v. Q1 = 0·35, 95 % CI = 0·27, 0·44, P < 0·001) and PhenoAgeAccel (βQ4 v. Q1 = 0·32, 95 % CI = 0·23, 0·41, P < 0·001). Each 1-SD increase in SMD score was positively associated with the acceleration of BA by 7·90 % for KDMAccel (P < 0·001) and 7·80 % for PhenoAgeAccel (P < 0·001). BMI and AST/ALT mediated the association. The stratified analysis revealed stronger accelerated ageing impacts in males and smokers. Our study indicated a higher SMD score is associated with elevated markers of biological ageing, supporting the potential utility of gut microbiota-targeted dietary interventions in attenuating the ageing process.

Optical fibers offer convenient access to a variety of nonlinear phenomena. However, due to their inversion symmetry, second-order nonlinear effects, such as second-harmonic generation (SHG), are challenging to achieve. Here, all-fiber in-core SHG with high beam quality is achieved in a random fiber laser (RFL). The fundamental wave (FW) is generated in the same RFL. The phase-matching condition is mainly achieved through an induced periodic electric field and the gain is enhanced through the passive spatiotemporal gain modulation and the extended fiber. The conversion needs no pretreatment and the average second-harmonic (SH) power reaches up to 10.06 mW, with a corresponding conversion efficiency greater than 0.04%. Moreover, a theoretical model is constructed to explain the mechanism and simulate the evolution of the SH and FW. Our work offers a simple method to generate higher brightness for in-fiber SHs, and may further provide new directions for research on all-fiber χ(2)-based nonlinear fiber optics and RFLs.

In certain scenarios, the large footprint of a robot is not conducive to multi-robot cooperative operations. This paper presents a generalized single-loop parallel manipulator with remote center of motion (GSLPM-RCM), which addresses this issue by incorporating a reconfigurable base. The footprint of this RCM manipulator can be adjusted by varying the parameters of the reconfigurable base. First, utilizing configuration evolution, a reconfigurable base is constructed based on the principle of forming RCM motion. Then, according to the modular analysis method, the inverse kinematics of this parallel RCM manipulator is analyzed, and the workspace is also analyzed. Subsequently, the motion/force transmissibility of this RCM manipulator is analyzed by considering its single-loop and multi-degree of freedom characteristics. Leveraging the workspace index and transmissibility indices, dimension optimization of the manipulator is implemented. Finally, the influence of the reconfigurable base on the workspace and the transmissibility performance of the optimized manipulator is studied.

Foodborne diseases are ongoing and significant public health concerns. This study analysed data obtained from the Foodborne Outbreaks Surveillance System of Wenzhou to comprehensively summarise the characteristics of foodborne outbreaks from 2012 to 2022. A total of 198 outbreaks were reported, resulting in 2,216 cases, 208 hospitalisations, and eight deaths over 11 years. The findings suggested that foodborne outbreaks were more prevalent in the third quarter, with most cases occurring in households (30.8%). Outbreaks were primarily associated with aquatic products (17.7%) as sources of contamination. The primary transmission pathways were accidental ingestion (20.2%) and multi-pathway transmission (12.1%). Microbiological aetiologies (46.0%), including Vibrio parahaemolyticus, Salmonella ssp., and Staphylococcus aureus, were identified as the main causes of foodborne outbreaks. Furthermore, mushroom toxins (75.0%), poisonous animals (12.5%), and poisonous plants (12.5%) were responsible for deaths from accidental ingestion. This study identified crucial settings and aetiologies that require the attention of both individuals and governments, thereby enabling the development of effective preventive measures to mitigate foodborne outbreaks, particularly in coastal cities.

Sjögren's syndrome (SS) is a chronic autoimmune disease caused by immune system disorders. The main clinical manifestations of SS are dry mouth and eyes caused by the destruction of exocrine glands, such as the salivary and lacrimal glands, and systemic manifestations, such as interstitial pneumonia, interstitial nephritis and vasculitis. The pathogenesis of this condition is complex. However, this has not been fully elucidated. Treatment mainly consists of glucocorticoids, disease-modifying antirheumatic drugs and biological agents, which can only control inflammation but not repair the tissue. Therefore, identifying methods to regulate immune disorders and repair damaged tissues is imperative. Cell therapy involves the transplantation of autologous or allogeneic normal or bioengineered cells into the body of a patient to replace damaged cells or achieve a stronger immunomodulatory capacity to cure diseases, mainly including stem cell therapy and immune cell therapy. Cell therapy can reduce inflammation, relieve symptoms and promote tissue repair and regeneration of exocrine glands such as the salivary glands. It has broad application prospects and may become a new treatment strategy for patients with SS. However, there are various challenges in cell preparation, culture, storage and transportation. This article reviews the research status and prospects of cell therapies for SS.

MicroRNAs (miRNAs) are endogenous, non-coding RNAs, which are functional in a variety of biological processes through post-transcriptional regulation of gene expression. However, the role of miRNAs in the interaction between Bacillus thuringiensis and insects remains unclear. In this study, small RNA libraries were constructed for B. thuringiensis-infected (Bt) and uninfected (CK) Spodoptera exigua larvae (treated with double-distilled water) using Illumina sequencing. Utilising the miRDeep2 and Randfold, a total of 233 known and 726 novel miRNAs were identified, among which 16 up-regulated and 34 down-regulated differentially expressed (DE) miRNAs were identified compared to the CK. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed that potential target genes of DE miRNAs were associated with ABC transporters, fatty acid metabolism and MAPK signalling pathway which are related to the development, reproduction and immunity. Moreover, two miRNA core genes, SeDicer1 and SeAgo1 were identified. The phylogenetic tree showed that lepidopteran Dicer1 clustered into one branch, with SeDicer1 in the position closest to Spodoptera litura Dicer1. A similar phylogenetic relationship was observed in the Ago1 protein. Expression of SeDicer1 increased at 72 h post infection (hpi) with B. thuringiensis; however, expression of SeDicer1 and SeAgo1 decreased at 96 hpi. The RNAi results showed that the knockdown of SeDicer1 directly caused the down-regulation of miRNAs and promoted the mortality of S. exigua infected by B. thuringiensis GS57. In conclusion, our study is crucial to understand the relationship between miRNAs and various biological processes caused by B. thuringiensis infection, and develop an integrated pest management strategy for S. exigua via miRNAs.

Autoimmune diseases are pathological autoimmune reactions in the body caused by various factors, which can lead to tissue damage and organ dysfunction. They can be divided into organ-specific and systemic autoimmune diseases. These diseases usually involve various body systems, including the blood, muscles, bones, joints and soft tissues. The transient receptor potential (TRP) and PIEZO receptors, which resulted in David Julius and Ardem Patapoutian winning the Nobel Prize in Physiology or Medicine in 2021, attracted people's attention. Most current studies on TRP and PIEZO receptors in autoimmune diseases have been carried out on animal model, only few clinical studies have been conducted. Therefore, this study aimed to review existing studies on TRP and PIEZO to understand the roles of these receptors in autoimmune diseases, which may help elucidate novel treatment strategies.

Lymph node tuberculosis is particularly common in regions with a high tuberculosis burden, and it has a great risk of rupture. This study aims to investigate the utility of ultrasound multimodal imaging in predicting the rupture of cervical tuberculous lymphadenitis (CTL). 128 patients with unruptured CTL confirmed by pathology or laboratory tests were included. Various ultrasonic image features, including long-to-short-axis ratio (L/S), margin, internal echotexture, coarse calcification, Color Doppler Flow Imaging (CDFI), perinodal echogenicity, elastography score, and non-enhanced area proportion in contrast-enhanced ultrasound (CEUS), were analyzed to determine their predictive value for CTL rupture within a one-year follow-up period. As a result, L/S (P < 0.001), margin (P < 0.001), internal echotexture (P < 0.001), coarse calcification (P < 0.001), perinodal echogenicity (P < 0.001), and the area of non-enhancement in CEUS (P < 0.001) were identified as significant imaging features for predicting CTL rupture. The prognostic prediction showed a sensitivity of 89.29%, specificity of 100%, accuracy of 95.31%, respectively. Imaging findings such as L/S < 2, unclear margin, heterogeneous internal echotexture, perinodal echogenicity changed, and non-enhancement area in CEUS > 1/2, are indicative of CTL rupture, while coarse calcification in the lymph nodes is associated with a favorable prognosis.

The efficient separation of hexane isomers from the light naphtha fraction is a significant challenge in the petrochemical industry. 5A zeolite adsorbent is used commercially to sieve alkane isomers. In this study, 5A zeolites were synthesized using a low-cost natural clay mineral precursor, i.e. palygorskite (PAL), with the addition of crystallization directing agent (CDA). By varying the mass ratio of CDA/deionized water, 5A zeolites were obtained as CDA-5%, CDA-7.5%, and CDA-10%. All products were submicron particles with an average particle size of 400–800 nm. A sieving test of CDA-induced 5A zeolites was carried out on hexane adsorbates including n-hexane (nHEX), 2-methylpentane (2MP), and 3-methylpentane (3MP). According to vapor-phase batch adsorption experiments, a significant equilibrium amount (0.149 g/g) of nHEX and only 0.0321 g/g 2MP and 0.0416 g/g 3MP were adsorbed on the 5A zeolite product with CDA-5%. The dynamic adsorption performance of 5A zeolite (CDA-5%) was evaluated by breakthrough curves of binary mixtures of nHEX/2MP and nHEX/3MP. Palygorskite 5A (PAL 5A) zeolite achieved maximum dynamic adsorption capacities of nHEX (0.16 g/g in both cases) at 200°C and 1.2 MPa total pressure. This work provided an economic alternative for the synthesis of 5A zeolites using natural clay minerals instead of chemical raw materials.