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Random amplified polymorphic DNA technique for the identification of Trichinella species

Published online by Cambridge University Press:  06 April 2009

C. Bandi ,
G. La Rosa ,
S. Comincini ,
G. Damiani  and
E. Pozio
C. Bandi
Affiliation:
Istituto di Patologia Generate Veterinaria, Università di Milano, Milan, Italy
G. La Rosa
Affiliation:
Laboratory of Parasitology, Istituto Superiore di Sanità, viale R. Elena 299, 00161 Rome, Italy
S. Comincini
Affiliation:
I.D.V.G.A.-C.N.R., Milan, Italy
G. Damiani
Affiliation:
I.D.V.G.A.-C.N.R., Milan, Italy
E. Pozio
Affiliation:
Laboratory of Parasitology, Istituto Superiore di Sanità, viale R. Elena 299, 00161 Rome, Italy
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Summary

The random amplified polymorphic DNA (RAPD) technique was successfully used to produce genetic fingerprints distinguishing between Trichinella spiralis and Trichinella britovi. The same patterns were obtained from purified and crude DNA preparations of pooled and single muscle larvae. RAPD fingerprinting was applied to muscle larvae preserved under different conditions and recovered from different hosts. Larvae recovered from fresh and frozen meat and stored at – 20 °C for a long time or under 70% ethyl alcohol at room temperature for 30 d gave good and reproducible results. Single larvae recovered from a naturally infected wild boar and from a human biopsy gave fingerprints congruent to those obtained from T. britovi reference strains. The results prove that RAPD analysis is a quick method to distinguish between the autochthonous Trichinella species of Central-Southern Europe in less than 1 d after the detection of the infection. If necessary, the biological material can be frozen or stored under 70% ethyl alcohol at room temperature and sent to laboratories able to perform the RAPD analysis. The RAPD technique requires no prior knowledge of the molecular biology of the organism to be investigated and therefore appears to be a promising tool in parasitology for the identification of sibling species.

Keywords

Trichinella spiralisTrichinella britovirandom amplified polymorphic DNARAPDtaxonomyepidemiologymuscle larva

Information

Type
Research Article
Information
Parasitology , Volume 107 , Issue 4 , November 1993 , pp. 419 - 424
Copyright
Copyright © Cambridge University Press 1993

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