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Oxidative stress and changes in adenosine deaminase activity of cattle experimentally infected by Fasciola hepatica

Published online by Cambridge University Press:  20 January 2017

ALEKSANDRO S. DA SILVA ,
MATHEUS D. BALDISSERA ,
NATHIELI B. BOTTARI ,
MATHEUS E. GABRIEL ,
LEANDRO A. RHODEN ,
MANOELA M. PIVA ,
RICARDO CHRIST ,
FERNANDA A. STEDILLE ,
ANDERSON GRIS  and
VERA M. MORSCH
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ALEKSANDRO S. DA SILVA*
Affiliation:
Graduate Program in Animal Science, Universidade do Estado de Santa Catarina (UDESC), Chapecó, Brazil Department of Biochemistry and Molecular Biology and Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Brazil
MATHEUS D. BALDISSERA
Affiliation:
Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Brazil
NATHIELI B. BOTTARI
Affiliation:
Department of Biochemistry and Molecular Biology and Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Brazil
MATHEUS E. GABRIEL
Affiliation:
Laboratory of Veterinary Pathology, Instituto Federal Catarinense (IFC), Concórdia, SC, Brazil
LEANDRO A. RHODEN
Affiliation:
Laboratory of Veterinary Pathology, Instituto Federal Catarinense (IFC), Concórdia, SC, Brazil
MANOELA M. PIVA
Affiliation:
Laboratory of Veterinary Pathology, Instituto Federal Catarinense (IFC), Concórdia, SC, Brazil
RICARDO CHRIST
Affiliation:
Laboratory of Veterinary Pathology, Instituto Federal Catarinense (IFC), Concórdia, SC, Brazil
FERNANDA A. STEDILLE
Affiliation:
Laboratory of Veterinary Pathology, Instituto Federal Catarinense (IFC), Concórdia, SC, Brazil
ANDERSON GRIS
Affiliation:
Laboratory of Veterinary Pathology, Instituto Federal Catarinense (IFC), Concórdia, SC, Brazil
VERA M. MORSCH
Affiliation:
Department of Biochemistry and Molecular Biology and Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Brazil
MARIA ROSA SCHETINGER
Affiliation:
Department of Biochemistry and Molecular Biology and Department of Microbiology and Parasitology, Universidade Federal de Santa Maria (UFSM), Brazil
RICARDO E. MENDES*
Affiliation:
Laboratory of Veterinary Pathology, Instituto Federal Catarinense (IFC), Concórdia, SC, Brazil
*
*Corresponding authors: UDESC, Animal Science, Rua Beloni Trombeta Zanini, Chapecó 89805-030, Brazil. E-mail: aleksandro_ss@yahoo.com.br; ricardo.mendes@ifc-concordia.edu.br
*Corresponding authors: UDESC, Animal Science, Rua Beloni Trombeta Zanini, Chapecó 89805-030, Brazil. E-mail: aleksandro_ss@yahoo.com.br; ricardo.mendes@ifc-concordia.edu.br
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Summary

The aim of this study was to evaluate the oxidative stress in serum and liver and adenosine deaminase (ADA) activity of cattle experimentally infected by Fasciola hepatica. The group A consisted of five healthy animals (uninfected), and the group B was composed of five animals orally infected with 200 metacercariae of F. hepatica. On days 20, 40, 60 and 80 post-infection (PI) serum was collected to measure oxidative stress variables. On day 100 PI, animals were humanely euthanized and liver samples were collected. Infected animals showed lower (P < 0·05) seric ADA activities on days 40 and 60 PI but higher (P < 0·05) in the liver tissue compared with uninfected animals. Seric and hepatic reactive oxygen species (ROS) were higher (P < 0·05) in infected compared with uninfected animals. Hepatic thiobarbituric acid reactive substances were higher (P < 0·05) in infected animals. Catalase and glutathione S-transferase activities were lower in liver tissue of infected animals, while glutathione peroxidase was higher compared with uninfected (P < 0·05). In summary, we conclude that oxidative stress occurs in cattle experimentally infected by F. hepatica, mainly due to excessive ROS production in the course of fasciolosis, contributing to hepatic damage, and that increased in hepatic ADA activity may contribute to the inflammatory process.

Keywords

ADAfasciolosisoxidative stresszoonosis

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Type
Research Article
Information
Parasitology , Volume 144 , Issue 4 , April 2017 , pp. 520 - 526
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Copyright © Cambridge University Press 2017 

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