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Echinococcus metacestodes as laboratory models for the screening of drugs against cestodes and trematodes

Published online by Cambridge University Press:  21 September 2009

A. HEMPHILL ,
B. STADELMANN ,
S. SCHOLL ,
J. MÜLLER ,
M. SPILIOTIS ,
N. MÜLLER ,
B. GOTTSTEIN  and
M. SILES-LUCAS
A. HEMPHILL*
Affiliation:
Institute of Parasitology, University of Berne, Laenggass-Strasse 122, CH-3012Berne, Switzerland
B. STADELMANN
Affiliation:
Institute of Parasitology, University of Berne, Laenggass-Strasse 122, CH-3012Berne, Switzerland
S. SCHOLL
Affiliation:
Institute of Parasitology, University of Berne, Laenggass-Strasse 122, CH-3012Berne, Switzerland
J. MÜLLER
Affiliation:
Institute of Parasitology, University of Berne, Laenggass-Strasse 122, CH-3012Berne, Switzerland
M. SPILIOTIS
Affiliation:
Institute of Parasitology, University of Berne, Laenggass-Strasse 122, CH-3012Berne, Switzerland
N. MÜLLER
Affiliation:
Institute of Parasitology, University of Berne, Laenggass-Strasse 122, CH-3012Berne, Switzerland
B. GOTTSTEIN
Affiliation:
Institute of Parasitology, University of Berne, Laenggass-Strasse 122, CH-3012Berne, Switzerland
M. SILES-LUCAS
Affiliation:
Parasitology Laboratory, Instituto de Recursos Naturales y Agrobiología de Salamanca (CSIC), Cordel de Merinas 40-52, 37008Salamanca, Spain
*
*Corresponding author: Andrew Hemphill, Institute of Parasitology Vetsuisse Faculty, University of BerneLänggass-Strasse 122, CH-3012Berne, Switzerland. Tel: +41 31 6312384. Fax: +41 31 6312477. Email: hemphill@ipa.unibe.ch
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Summary

Among the cestodes, Echinococcus granulosus, Echinococcus multilocularis and Taenia solium represent the most dangerous parasites. Their larval stages cause the diseases cystic echinococcosis (CE), alveolar echincoccosis (AE) and cysticercosis, respectively, which exhibit considerable medical and veterinary health concerns with a profound economic impact. Others caused by other cestodes, such as species of the genera Mesocestoides and Hymenolepis, are relatively rare in humans. In this review, we will focus on E. granulosus and E. multilocularis metacestode laboratory models and will review the use of these models in the search for novel drugs that could be employed for chemotherapeutic treatment of echinococcosis. Clearly, improved therapeutic drugs are needed for the treatment of AE and CE, and this can only be achieved through the development of medium-to-high throughput screening approaches. The most recent achievements in the in vitro culture and genetic manipulation of E. multilocularis cells and metacestodes, and the accessability of the E. multilocularis genome and EST sequence information, have rendered the E. multilocularis model uniquely suited for studies on drug-efficacy and drug target identification. This could lead to the development of novel compounds for the use in chemotherapy against echinococcosis, and possibly against diseases caused by other cestodes, and potentially also trematodes.

Keywords

Cystic echinococcosis (CE)alveolar echinococcosis (AE)Echinoccoccus granulosusEchinoccoccus multilocularisin vitro culturechemotherapy

Information

Type
Research Article
Information
Parasitology , Volume 137 , Special Issue 3: Development and applications of cestode and trematode laboratory models , March 2010 , pp. 569 - 587
Copyright
Copyright © Cambridge University Press 2009

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