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Diversity of Cryptosporidium in common voles and description of Cryptosporidium alticolis sp. n. and Cryptosporidium microti sp. n. (Apicomplexa: Cryptosporidiidae)

Published online by Cambridge University Press:  17 July 2018

Michaela Horčičková ,
Šárka Čondlová ,
Nikola Holubová ,
Bohumil Sak ,
Dana Květoňová ,
Lenka Hlásková ,
Roman Konečný ,
František Sedláček ,
Mark Clark  and
Catherine Giddings
...Show all authors
Michaela Horčičková
Affiliation:
Faculty of Agriculture, University of South Bohemia in České Budějovice, Czech Republic Institute of Parasitology, Biology Centre of the Academy of Sciences of the Czech Republic, České Budějovice, Czech Republic
Šárka Čondlová
Affiliation:
Faculty of Agriculture, University of South Bohemia in České Budějovice, Czech Republic Institute of Parasitology, Biology Centre of the Academy of Sciences of the Czech Republic, České Budějovice, Czech Republic
Nikola Holubová
Affiliation:
Faculty of Agriculture, University of South Bohemia in České Budějovice, Czech Republic Institute of Parasitology, Biology Centre of the Academy of Sciences of the Czech Republic, České Budějovice, Czech Republic
Bohumil Sak
Affiliation:
Institute of Parasitology, Biology Centre of the Academy of Sciences of the Czech Republic, České Budějovice, Czech Republic
Dana Květoňová
Affiliation:
Institute of Parasitology, Biology Centre of the Academy of Sciences of the Czech Republic, České Budějovice, Czech Republic
Lenka Hlásková
Affiliation:
Institute of Parasitology, Biology Centre of the Academy of Sciences of the Czech Republic, České Budějovice, Czech Republic
Roman Konečný
Affiliation:
Faculty of Agriculture, University of South Bohemia in České Budějovice, Czech Republic
František Sedláček
Affiliation:
Faculty of Science, University of South Bohemia in České Budějovice, Czech Republic
Mark Clark
Affiliation:
Biological Sciences Department, North Dakota State University, Fargo, USA
Catherine Giddings
Affiliation:
Microbiological Sciences Department, North Dakota State University, Fargo, USA
John McEvoy
Affiliation:
Microbiological Sciences Department, North Dakota State University, Fargo, USA
Martin Kváč*
Affiliation:
Faculty of Agriculture, University of South Bohemia in České Budějovice, Czech Republic Institute of Parasitology, Biology Centre of the Academy of Sciences of the Czech Republic, České Budějovice, Czech Republic
*
Author for correspondence: M. Kváč, E-mail: kvac@paru.cas.cz
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Abstract

Fecal samples from wild-caught common voles (n = 328) from 16 locations in the Czech Republic were screened for Cryptosporidium by microscopy and PCR/sequencing at loci coding small-subunit rRNA, Cryptosporidium oocyst wall protein, actin and 70 kDa heat shock protein. Cryptosporidium infections were detected in 74 voles (22.6%). Rates of infection did not differ between males and females nor between juveniles and adults. Phylogenetic analysis revealed the presence of eight Cryptosporidium species/genotypes including two new species, C. alticolis and C. microti. These species from wild-caught common voles were able to infect common and meadow voles under experimental conditions, with a prepatent period of 3–5 days post-infection (DPI), but they were not infectious for various other rodents or chickens. Meadow voles lost infection earlier than common voles (11–14 vs 13–16 DPI) and had significantly lower infection intensity. Cryptosporidium alticolis infects the anterior small intestine and has larger oocysts (5.4 × 4.9 µm), whereas C. microti infects the large intestine and has smaller oocysts (4.3 × 4.1 µm). None of the rodents developed clinical signs of infection. Genetic and biological data support the establishment of C. alticolis and C. microti as separate species of the genus Cryptosporidium.

Keywords

Experimental infectionmolecular analysesoocyst sizephylogenyRodentiavoles

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Research Article
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Parasitology , Volume 146 , Issue 2 , February 2019 , pp. 220 - 233
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Copyright © Cambridge University Press 2018 

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