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3-Dimensional graphene/Cu/Fe3O4 composites: Immobilized laccase electrodes for detecting bisphenol A

Published online by Cambridge University Press:  04 September 2019

Congqiang Lou ,
Tao Jing ,
Jingzhi Tian ,
Yongjie Zheng ,
Jiaoxia Zhang ,
Mengyao Dong ,
Chao Wang ,
Chuanxin Hou ,
Jincheng Fan  and
Zhanhu Guo
Congqiang Lou
Affiliation:
School of Chemistry and Chemical Engineering, Qiqihar University, Qiqihar, Heilongjiang Province 161000, China
Tao Jing*
Affiliation:
School of Chemistry and Chemical Engineering, Qiqihar University, Qiqihar, Heilongjiang Province 161000, China
Jingzhi Tian
Affiliation:
School of Chemistry and Chemical Engineering, Qiqihar University, Qiqihar, Heilongjiang Province 161000, China
Yongjie Zheng*
Affiliation:
School of Chemistry and Chemical Engineering, Qiqihar University, Qiqihar, Heilongjiang Province 161000, China
Jiaoxia Zhang*
Affiliation:
School of Materials Science and Engineering, Jiangsu University of Science and Technology, Zhenjiang 212003, China
Mengyao Dong
Affiliation:
Key Laboratory of Materials Processing and Mold (Zhengzhou University), Ministry of Education, National Engineering Research Center for Advanced Polymer Processing Technology, Zhengzhou University, Zhengzhou 450001, China
Chao Wang
Affiliation:
School of Chemistry and Chemical Engineering, Qiqihar University, Qiqihar, Heilongjiang Province 161000, China
Chuanxin Hou
Affiliation:
Integrated Composites Laboratory (ICL), Department of Chemical & Biomolecular Engineering, University of Tennessee, Knoxville, Tennessee 37996, USA; and School of Materials Science and Engineering, North University of China, Taiyuan 030051, China
Jincheng Fan
Affiliation:
College of Materials Science and Engineering, Changsha University of Science and Technology, Changsha 410114, China
Zhanhu Guo*
Affiliation:
Integrated Composites Laboratory (ICL), Department of Chemical & Biomolecular Engineering, University of Tennessee, Knoxville, Tennessee 37996, USA
*
a)Address all correspondence to these authors. e-mail: jtkr@163.com
b)e-mail: zyj1964@163.com
c) e-mail: zhangjx@just.edu.cn
d)e-mail: zguo10@utk.edu
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Abstract

Three-dimensional graphene (3D-GN)/Cu/Fe3O4 composite support materials were synthesized by a modified chemical reduction method using graphene oxide precursor. A 3D-GN/Cu/Fe3O4 biosensor was prepared by coating the electrode with laccase. The electrochemical properties of the biosensor were investigated by cyclic voltammetry (CV) and differential pulse voltammetry using potassium ferricyanide, phosphate-buffered saline (PBS) solution, and bisphenol A (BPA) solution. The current response of 3D-GN/Cu/Fe3O4 biosensors presents a remarkable sensitivity based on CV. The linear range of BPA is 7.2–18 μM using differential pulse voltammetry in PBS solution (pH = 4.0). A linear fitting equation of the laccase biosensor was observed for the current response as a function of BPA concentration. The detection limit was decreased to 1.7 μM. The detection approach herein turns out to be highly sensitive, has a wide linear range, and exhibits excellent stability.

Keywords

sensorelectrical propertiesgraphene

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