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Sequence variability in two mitochondrial DNA regions and internal transcribed spacer among three cestodes infecting animals and humans from China

Published online by Cambridge University Press:  12 July 2011

R.S. Dai ,
G.H. Liu ,
H.Q. Song ,
R.Q. Lin ,
Z.G. Yuan ,
M.W. Li ,
S.Y. Huang ,
W. Liu  and
X.Q. Zhu
R.S. Dai
Affiliation:
College of Veterinary Medicine, Hunan Agricultural University, Changsha, Hunan Province410128, PR China State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, CAAS, Lanzhou, Gansu Province730046, PR China
G.H. Liu
Affiliation:
College of Veterinary Medicine, Hunan Agricultural University, Changsha, Hunan Province410128, PR China State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, CAAS, Lanzhou, Gansu Province730046, PR China
H.Q. Song
Affiliation:
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, CAAS, Lanzhou, Gansu Province730046, PR China College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province510642, PR China
R.Q. Lin
Affiliation:
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, CAAS, Lanzhou, Gansu Province730046, PR China College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province510642, PR China
Z.G. Yuan
Affiliation:
College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong Province510642, PR China
M.W. Li
Affiliation:
Department of Veterinary Medicine, Guangdong Ocean University, Zhanjiang, Guangdong Province524088, PR China
S.Y. Huang
Affiliation:
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, CAAS, Lanzhou, Gansu Province730046, PR China
W. Liu*
Affiliation:
College of Veterinary Medicine, Hunan Agricultural University, Changsha, Hunan Province410128, PR China State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, CAAS, Lanzhou, Gansu Province730046, PR China
X.Q. Zhu*
Affiliation:
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, CAAS, Lanzhou, Gansu Province730046, PR China College of Animal Science and Technology, Yunnan Agricultural University, Kunming, Yunnan Province650201, PR China
*
*Fax: +86 (931) 8340977 E-mail: xingquanzhu1@hotmail.com (X.Q.Z.); E-mail: liuwpro@163.com (W.L.)
*Fax: +86 (931) 8340977 E-mail: xingquanzhu1@hotmail.com (X.Q.Z.); E-mail: liuwpro@163.com (W.L.)
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Abstract

Sequence variability in two mitochondrial DNA (mtDNA) regions, namely cytochrome c oxidase subunit 1 (cox1) and NADH dehydrogenase subunit 4 (nad4), and internal transcribed spacer (ITS) of rDNA among and within three cestodes, Spirometra erinaceieuropaei, Taenia multiceps and Taenia hydatigena, from different geographical origins in China was examined. A portion of the cox1 (pcox1), nad4 genes (pnad4) and the ITS (ITS1+5.8S rDNA+ITS2) were amplified separately from individual cestodes by polymerase chain reaction (PCR). Representative amplicons were subjected to sequencing in order to estimate sequence variability. While the intra-specific sequence variations within each of the tapeworm species were 0–0.7% for pcox1, 0–1.7% for pnad4 and 0.1–3.6% for ITS, the inter-specific sequence differences were significantly higher, being 12.1–17.6%, 18.7–26.2% and 31–75.5% for pcox1, pnad4 and ITS, respectively. Phylogenetic analyses based on the pcox1 sequence data revealed that T. multiceps and T. hydatigena were more closely related to the other members of the Taenia genus, and S. erinaceieuropaei was more closely related to the other members of the Spirometra genus. These findings demonstrated clearly the usefulness of mtDNA and rDNA sequences for population genetic studies of these cestodes of socio-economic importance.

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Type
Research Papers
Information
Journal of Helminthology , Volume 86 , Issue 2 , June 2012 , pp. 245 - 251
Copyright
Copyright © Cambridge University Press 2011

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