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Interactions between Trichinella spiralis infection and induced colitis in mice

Published online by Cambridge University Press:  12 February 2013

D.S. Ashour ,
A.A. Othman ,
M.M. Shareef ,
H.H. Gaballah  and
W.W. Mayah
D.S. Ashour
Affiliation:
Department of Medical Parasitology, Tanta Faculty of Medicine, Egypt
A.A. Othman*
Affiliation:
Department of Medical Parasitology, Tanta Faculty of Medicine, Egypt
M.M. Shareef
Affiliation:
Department of Pathology, Tanta Faculty of Medicine, Egypt
H.H. Gaballah
Affiliation:
Department of Biochemistry, Tanta Faculty of Medicine, Egypt
W.W. Mayah
Affiliation:
Department of Tropical Medicine, Tanta Faculty of Medicine, Egypt
*
*Fax: 002 40 34 07734 E-mail: ahmed_ali44@hotmail.com
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Abstract

Inflammatory bowel disease (IBD) is a chronic relapsing inflammation afflicting any part of the bowel wall as a result of a deregulated and inappropriate immune response. In recent years, experimental and clinical evidence has demonstrated that infection with parasitic worms could protect hosts from IBD. The aims of this study were to determine if the underlying mechanism of the host immune regulation inherent to Trichinella spiralis infection involves Foxp3-expressing regulatory T cells, and to gain insight about time-related interactions between intestinal nematode infection and induced colitis using an experimental model for ulcerative colitis. Mice were experimentally subjected to acetic acid-induced colitis, which was either preceded or followed by T. spiralis infection. Assessment of colitis was done by histopathological examination of the colon and determination of pentraxin 3 levels. Immunohistochemistry was done for demonstration of Foxp3-expressing regulatory T cells in colonic tissues. It was evident that T. spiralis infection ameliorated the severe inflammation induced by acetic acid, evidenced by amelioration of histopathological changes and diminution of pentraxin 3 levels. The amelioration was more pronounced when T. spiralis infection preceded the induction of colitis. Regarding the immunohistochemical staining of regulatory T cells, T. spiralis infection induced recruitment of Foxp3-expressing regulatory T cells to areas of inflammation. In conclusion, T. spiralis regulatory mechanism can improve inflammation of the colon through the ‘inflammatory–regulatory’ axis. Finally, it would be of great importance to apply these results to the development of new therapeutic approaches for the treatment of ulcerative colitis.

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Type
Research Papers
Information
Journal of Helminthology , Volume 88 , Issue 2 , June 2014 , pp. 210 - 218
Copyright
Copyright © Cambridge University Press 2013 

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