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Canine echinococcosis: genetic diversity of Echinococcus granulosus sensu stricto (s.s.) from definitive hosts

Published online by Cambridge University Press:  16 July 2015

B. Boufana ,
W. Lett ,
S. Lahmar ,
A. Griffiths ,
D.J. Jenkins ,
I. Buishi ,
S.A. Engliez ,
M.A. Alrefadi ,
A.A. Eljaki  and
F.M. Elmestiri
...Show all authors
B. Boufana*
Affiliation:
Cestode Zoonoses Research Group, School of Environment and Life Sciences, University of Salford, M5 4WT, United Kingdom
W. Lett
Affiliation:
Cestode Zoonoses Research Group, School of Environment and Life Sciences, University of Salford, M5 4WT, United Kingdom
S. Lahmar
Affiliation:
Parasitology Laboratory, National School of Veterinary Medicine, Sidi Thabet, Tunisia
A. Griffiths
Affiliation:
School of Biological Sciences, University of Bristol, Life Sciences Building, Bristol, BS8 1TQ, UK
D.J. Jenkins
Affiliation:
School of Animal and Veterinary Sciences, Charles Sturt University, NSW2678, Australia
I. Buishi
Affiliation:
The University of Tripoli, PO Box 606, Tripoli, Libya
S.A. Engliez
Affiliation:
Department of Zoology, University of Benghazi, PO Box 1308, Benghazi, Libya
M.A. Alrefadi
Affiliation:
Department of Zoology, University of Benghazi, PO Box 1308, Benghazi, Libya
A.A. Eljaki
Affiliation:
Department of Zoology, University of Omar Almukhtar, Al Bayda, Libya
F.M. Elmestiri
Affiliation:
Department of Zoology, University of Benghazi, PO Box 1308, Benghazi, Libya
M.M. Reyes
Affiliation:
Alberto Hurtado School of Medicine, Universidad Peruana Cayetano Heredia, Lima, Peru
S. Pointing
Affiliation:
Department of Agriculture, Stanley, Falkland Islands, FIQQ 1ZZ
A. Al-Hindi
Affiliation:
The Islamic University of Gaza, Faculty of Health Sciences, Medical Laboratory Sciences Department, Gaza, Palestine
P.R. Torgerson
Affiliation:
Section of Veterinary Epidemiology, University of Zürich, Switzerland
M. Okamoto
Affiliation:
Primate Research Institute, Kyoto University, 41-2 Kanrin, Inuyama, Aichi 484-8506, Japan
P.S. Craig
Affiliation:
Cestode Zoonoses Research Group, School of Environment and Life Sciences, University of Salford, M5 4WT, United Kingdom
*
*E-mail: bboufana@yahoo.com
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Abstract

Canids, particularly dogs, constitute the major source of cystic echinococcosis (CE) infection to humans, with the majority of cases being caused by Echinococcus granulosus (G1 genotype). Canine echinococcosis is an asymptomatic disease caused by adult tapeworms of E. granulosus sensu lato (s.l.). Information on the population structure and genetic variation of adult E. granulosus is limited. Using sequenced data of the mitochondrial cytochrome c oxidase subunit 1 (cox1) we examined the genetic diversity and population structure of adult tapeworms of E. granulosus (G1 genotype) from canid definitive hosts originating from various geographical regions and compared it to that reported for the larval metacestode stage from sheep and human hosts. Echinococcus granulosus (s.s) was identified from adult tapeworm isolates from Kenya, Libya, Tunisia, Australia, China, Kazakhstan, United Kingdom and Peru, including the first known molecular confirmation from Gaza and the Falkland Islands. Haplotype analysis showed a star-shaped network with a centrally positioned common haplotype previously described for the metacestode stage from sheep and humans, and the neutrality indices indicated population expansion. Low Fst values suggested that populations of adult E. granulosus were not genetically differentiated. Haplotype and nucleotide diversities for E. granulosus isolates from sheep and human origin were twice as high as those reported from canid hosts. This may be related to self-fertilization of E. granulosus and/or to the longevity of the parasite in the respective intermediate and definitive hosts. Improved nuclear single loci are required to investigate the discrepancies in genetic variation seen in this study.

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Type
Research Papers
Information
Journal of Helminthology , Volume 89 , Issue 6 , November 2015 , pp. 689 - 698
Copyright
Copyright © Cambridge University Press 2015 

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