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Immunization of calves against the microfilariae of Onchocerca lienalis

Published online by Cambridge University Press:  18 November 2009

Simon Townson  and
A. E. Bianco
Simon Townson
Affiliation:
London School of Hygiene and Tropical Medicine, Winches Farm Field Station, 395 Hatfield Road, St Albans, Herts AL4 OXQ, England
A. E. Bianco
Affiliation:
London School of Hygiene and Tropical Medicine, Winches Farm Field Station, 395 Hatfield Road, St Albans, Herts AL4 OXQ, England
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Abstract

Three Jersey bull calves were immunized by subcutaneous injections of sonicated Onchocerca lienalis microfilariae suspended in phosphate buffered saline, and three control animals were injected with medium only. All calves received booster injections 27 days later, and were challenged with live microfilariae 44 days after the booster. Following challenge, only the immunized animals developed an elevated level of circulating eosinophils. When necropsied ten days after challenge there was a 97% reduction in recoveries of microfilariae from immunized animals compared to challenge controls. In human onchocerciasis it is the microfilariae which are the principal cause of pathology, and we believe that studies on O. lienalis in both the natural bovine host and in inbred rodents provide a promising model to investigate immunity to Onchocerca microfilariae.

Information

Type
Research Papers
Information
Journal of Helminthology , Volume 56 , Issue 4 , December 1982 , pp. 297 - 303
Copyright
Copyright © Cambridge University Press 1982

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