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Different chronological patterns of appearance of blood derived milk components during mastitis indicate different mechanisms of transfer from blood into milk

Published online by Cambridge University Press:  03 July 2015

Olga Wellnitz ,
Christina Zbinden ,
Johannes Lüttgenau ,
Heinrich Bollwein  and
Rupert M Bruckmaier
Olga Wellnitz
Affiliation:
Vetsuisse Faculty University of Zurich, Clinic of Reproductive Medicine, Switzerland
Christina Zbinden
Affiliation:
Vetsuisse Faculty University of Bern, Veterinary Physiology, Switzerland
Johannes Lüttgenau
Affiliation:
Vetsuisse Faculty University of Zurich, Clinic of Reproductive Medicine, Switzerland
Heinrich Bollwein
Affiliation:
Vetsuisse Faculty University of Zurich, Clinic of Reproductive Medicine, Switzerland
Rupert M Bruckmaier*
Affiliation:
Vetsuisse Faculty University of Bern, Veterinary Physiology, Switzerland
*
*For correspondence; e-mail: rupert.bruckmaier@vetsuisse.unibe.ch
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Abstract

This study aimed to describe chronological patterns of changes of various candidate blood components in milk during the acute phase of a mammary immune response in detail. Eight dairy cows were challenged with Escherichia coli lipopolysaccharide in one udder quarter. Milk from challenged and control quarters and blood samples were taken before, and 1 and 2 h after challenge and then every 15 min until 5 h after challenge. The SCC, serum albumin, immunoglobulin (Ig)G1, IgG2, lactate dehydrogenase (LDH), and L-lactate in milk and blood, and α-lactalbumin in blood were analysed. All selected parameters in milk increased in challenged quarters but did not increase in control quarters. Milk IgG1, IgG2, serum albumin, and LDH were already significantly increased at 2 h after challenge whereas a significant increase of SCC was detectable at 2·75 h and L-lactate was increased at 2·25 h after challenge. In blood L-lactate was increased at 3·75 h after challenge, however, other factors in blood did not change significantly within the 5 h of experiment. In conclusion, the increase of blood components in milk during inflammation follows two different patterns: There is a rapid increase for IgG1, IgG2, or LDH, before the increase of SCC, and their concentrations reach a plateau within 3 h. On the other hand, SCC and L-lactate show a slower but consistent increase not reaching a plateau within 5 h after LPS challenge. L-lactate increases to higher concentrations in milk than in blood. This clearly shows that the increase of blood components follows different patterns and is therefore a controlled and compound-specific process and not exclusively an unspecific type of leakage.

Keywords

dairy cowmastitisblood-milk barrier

Information

Type
Research Article
Information
Journal of Dairy Research , Volume 82 , Issue 3 , August 2015 , pp. 322 - 327
Copyright
Copyright © Proprietors of Journal of Dairy Research 2015 

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References

Baker, K, Quiao, SW, Kuo, T, Kobayashi, K, Yoshida, M, Lencer, WI & Blumberg, RS 2009 Immune and non-immune functions of the (not so) neonatal Fc receptor, FcRn. Seminars in Immunopathology 31 223–236CrossRefGoogle ScholarPubMed
Baumert, A, Bruckmaier, RM & Wellnitz, O, 2009a Dose dependant SCC changes after intramammary lipopolysaccharide challenge. Milk Science International 64 119–121Google Scholar
Baumert, A, Bruckmaier, RM & Wellnitz, O 2009b Cell population, viability, and some key immunomodulatory molecules in different milk somatic cell samples in dairy cows. Journal of Dairy Research 76 356–364CrossRefGoogle ScholarPubMed
Bruckmaier, RM, Schallibaum, M & Blum, JW 1993 Escherichia coli endotoxin-induced mastitis in dairy cows : changes and importance of insulin-like growth factor I and oxytocin. Milk Science International 48 374–378Google Scholar
Burton, JL & Erskine, RJ 2003 Immunity and mastitis. Some new ideas for an old disease. Veterinary Clinics Food Animal Practice 19 1–45CrossRefGoogle ScholarPubMed
Butler, JE 1983 Bovine immunoglobulins: an augmented review. Veterinary Immunology and Immunopathology 4 43–152CrossRefGoogle ScholarPubMed
Chagunda, MG, Larsen, T, Bjerring, M & Ingvartsen, KL 2006 L-lactate dehydrogenase and N-acetyl-beta-D-glucosaminidase activities in bovine milk as indicators of non-specific mastitis. Journal of Dairy Research 73 431–440CrossRefGoogle ScholarPubMed
Davis, SR, Farr, VC, Prosser, CG, Nicholas, GD, Turner, SA, Lee, J & Hart, AL 2004 Milk L-lactate concentration is increased during mastitis. Journal of Dairy Research 71 175–181CrossRefGoogle ScholarPubMed
Giri, SN, Emau, P, Cullor, JS, Stabenfeldt, GH, Bruss, ML, Bondurant, RH & Osburn, BI 1990 Effects of endotoxin infusion on circulating levels of eicosanoids, progesterone, cortisol, glucose and lactic acid, and abortion in pregnant cows. Veterinary Microbiology 21 211–231CrossRefGoogle ScholarPubMed
Glick, JH 1969 Serum lactate dehydrogenase isoenzyme and total lactate dehydrogenase values in health and disease, and clinical evaluation of these tests by means of discriminant analysis. American Journal of Clinical Pathology 52 320–328Google ScholarPubMed
Guidry, AJ, Ost, M, Mather, IH, Shainline, WE & Weinland, BT 1983 Sequential response of milk leukocytes, albumin, immunoglobulins, monovalent ions, citrate, and lactose in cows given infusions of Escherichia coli endotoxin into the mammary gland. American Journal of Veterinary Research 44 2262–2267Google ScholarPubMed
Harmon, RJ 1994 Physiology of mastitis and factors affecting somatic cell count. Journal of Dairy Science 77 2103–2112CrossRefGoogle Scholar
Harmon, RJ, Schanbacher, FL, Ferguson, LC & Smith, KL 1976 Changes in lactoferrin, immunoglobulin G, bovine serum albumin, and alpha-lactalbumin during acute experimental and natural coliform mastitis in cows. Infection and Immunity 13 533–542CrossRefGoogle ScholarPubMed
Kehrli, ME & Shuster, DE 1994 Factors affecting milk somatic cells and their role in health of the bovine mammary gland. Journal of Dairy Science 77 619–627CrossRefGoogle ScholarPubMed
Lehmann, M, Wellnitz, O & Bruckmaier, RM 2013 Concomitant lipopolysaccharide-induced transfer of blood-derived components including immunoglobulins into milk. Journal of Dairy Science 96 889–896CrossRefGoogle ScholarPubMed
Leitner, G, Yadlin, B, Glickman, A, Chaffer, M & Saran, A 2000 Systemic and local immune response of cows to intramammary infection with Staphylococcus aureus. Research in Veterinary Science 69 181–184CrossRefGoogle ScholarPubMed
McFadden, TB, Akers, RM & Capuco, AV 1988 Relationship of milk proteins in blood with somatic cell count in milk of dairy cows. Journal of Dairy Science 71 826–834CrossRefGoogle ScholarPubMed
Nguyen, DD & Neville, MC 1998 Tight junction regulation in the mammary gland. Journal of Mammary Gland Biology and Neoplasia 3 233–246CrossRefGoogle ScholarPubMed
Paape, MJ, Shafer-Weaver, K, Capuco, A, Van Oostveldt, K & Burvenich, C 2000 Immune surveillance of mammary tissues by phagocytic cells. Advances in Experimental Medicine and Biology 480 259–277CrossRefGoogle ScholarPubMed
Poutrel, B, Caffin, JP & Rainard, P 1983 Physiological and pathological factors influencing bovine serum albumin content of milk. Journal of Dairy Science 66 535–541CrossRefGoogle Scholar
Rainard, P & Caffin, JP 1983 Sequential changes in serum albumin, immunoglobulin (IgG1, IgG2, IgM) and lactoferrin concentrations in milk following infusion of Escherichia coli into the udder of immunized and unimmunized cows. Annales of Veterinary Research 14 271–279Google Scholar
Schmitz, S, Pfaffl, MW, Meyer, HHD & Bruckmaier, RM 2004 Short-term changes of mRNA expression of various inflammatory factors and milk proteins in mammary tissue during LPS-induced mastitis. Domestic Animal Endocrinology 26 111–126CrossRefGoogle ScholarPubMed
Shuster, DE, Kehrli, ME & Stevens, MG 1993 Cytokine production during endotoxin-induced mastitis in lactating dairy cows. American Journal of Veterinary Research 54 80–85Google ScholarPubMed
Silanikove, N, Rauch-Cohen, A, Shapiro, F, Blum, S, Arieli, A & Leitner, G 2011 Lipopolysaccharide challenge of the mammary gland in bovine induced a transient glandular shift to anaerobic metabolism. Journal of Dairy Science 94 4468–4475CrossRefGoogle ScholarPubMed
Shamay, A, Homans, R, Fuerman, Y, Levin, I, Barash, H, Silanikove, N & Mabjeesh, SJ 2005 Expression of albumin in nonhepatic tissues and its synthesis by the bovine mammary gland. Journal of Dairy Science 88 569–576CrossRefGoogle ScholarPubMed
Stelwagen, K, Politis, I, White, JH, Zavizion, B, Prosser, CG, Davis, SR & Farr, VC 1994 Effect of milking frequency and somatotropin on the activity of plasminogen activator, plasminogen, and plasma in bovine milk. Journal of Dairy Science 77 3577–3583CrossRefGoogle ScholarPubMed
Stelwagen, K, Farr, VC, McFadden, HA, Prosser, CG & Davis, SR 1997 Time course of milk accumulation-induced opening of mammary tight junctions, and blood clearance of milk components. American Journal of Physiology 273 R379–R386Google ScholarPubMed
Verhoeff, J & Smith, JA 1981 Bovine serum albumin and cell counts in the diagnosis of subclinical udder infections. Veterinary Quarterly 3 38–45CrossRefGoogle ScholarPubMed
Wellnitz, O & Bruckmaier, RM 2012 The innate immune response of the bovine mammary gland to bacterial infection. Veterinary Journal 192 148–152CrossRefGoogle ScholarPubMed
Wellnitz, O, Arnold, ET & Bruckmaier, RM 2011 Lipopolysaccharide and lipoteichoic acid induce different immune responses in the bovine mammary gland. Journal of Dairy Science 94 5405–5412CrossRefGoogle ScholarPubMed