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Molecular cloning, expression, and characterization of E2F transcription factor 4 from Antheraea pernyi

Published online by Cambridge University Press:  24 April 2017

M.N. Abbas ,
S. Kausar ,
Y.-X. Sun ,
Y. Sun ,
L. Wang ,
C. Qian ,
G.-Q. Wei ,
B.-J. Zhu  and
C.-L. Liu
M.N. Abbas
Affiliation:
College of Life Sciences, Anhui Agricultural University, Hefei 230036, China
S. Kausar
Affiliation:
College of Life Sciences, Anhui Agricultural University, Hefei 230036, China
Y.-X. Sun
Affiliation:
College of Life Sciences, Anhui Agricultural University, Hefei 230036, China
Y. Sun
Affiliation:
College of Life Sciences, Anhui Agricultural University, Hefei 230036, China
L. Wang
Affiliation:
College of Life Sciences, Anhui Agricultural University, Hefei 230036, China
C. Qian
Affiliation:
College of Life Sciences, Anhui Agricultural University, Hefei 230036, China
G.-Q. Wei
Affiliation:
College of Life Sciences, Anhui Agricultural University, Hefei 230036, China
B.-J. Zhu*
Affiliation:
College of Life Sciences, Anhui Agricultural University, Hefei 230036, China
C.-L. Liu*
Affiliation:
College of Life Sciences, Anhui Agricultural University, Hefei 230036, China
*
*Author for correspondence Phone: +86 551 6578-6201 Fax: +86 551 6578-6201 E-mail: zhubaojian@ahau.edu.cn and clliu@ahau.edu.cn
*Author for correspondence Phone: +86 551 6578-6201 Fax: +86 551 6578-6201 E-mail: zhubaojian@ahau.edu.cn and clliu@ahau.edu.cn
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Abstract

The E2F transcription factor family is distributed widely in eukaryotes and has been well studied among mammals. In the present study, the E2F transcription factor 4 (E2F4) gene was isolated from fat bodies of Antheraea pernyi and sequenced. E2F4 comprised a 795 bp open reading frame encoding a deduced amino acid sequence of 264 amino acid residues. The recombinant protein was expressed in Escherichia coli (Transetta DE3), and anti-E2F4 antibodies were prepared. The deduced amino acid sequence displayed significant homology to an E2F4-like protein from Bombyx mori L. Quantitative real-time polymerase chain reaction analysis revealed that E2F4 expression was highest in the integument, followed by the fat body, silk glands, and haemocytes. The expression of E2F4 was upregulated in larvae challenged by bacterial (Escherichia coli, Micrococcus luteus), viral (nuclear polyhedrosis virus), and fungal (Beauveria bassiana) pathogens. These observations indicated that E2F4 is an inducible protein in the immune response of A. pernyi and probably in other insects.

Keywords

quantitative real-time PCRsilkwormgenomehomology

Information

Type
Research Papers
Information
Bulletin of Entomological Research , Volume 107 , Issue 6 , December 2017 , pp. 839 - 846
Copyright
Copyright © Cambridge University Press 2017 

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